Construction Of Mutant Library By Mutator Insertionand Selection Of Drought Mutants In Maize

At present our country hybrid corn parents have less species, germplasm foundation is narrow, thecorn breeding process development is slow. Drought has become the main agricultural development of theimportant factors in the world, the mining of drought-resistant germplasm resources innovation, cultivatingnew varieties of corn drought is of great significance. But at present, the number of drought germplasmwere less corn, corn drought resistant breeding are greatly restricted. Therefore, the use of MU factor tobuild mutant pool maize germplasm resources, especially drought-resistant germplasm resources of theexpansion, improvement, to provide the good corn genetic breeding breeding excellent inbred lines.With the continuous development of sequencing technology, corn genome sequencing mass has beencompleted. Corn functional genomics is the study of each gene function and the biology mechanism. Theimportant method of studying maize functional genomics is to build corn mutant pool, mutant andcorresponding mutations has a causal relationship, can provide powerful evidence for elucidating thefunction of the gene.The method of constructing the mutant corn have many kinds, roughly divided into natural mutations,physical mutation, space mutagenesis, chemical mutagenesis, using the T-DNA insertional mutagenesis,using Mutator transposon tagging insertional mutagenesis, etc. Mu factor is corn genome endogenoustransposon tagging, has turned a highest activity, the most powerful mutation characteristics. Mutantnumber and type are rich.They are easy to observe. Use of MU factor construct mutant corn has becomeone of important ways of maize functional genomics research.115F for Mu transposon tagging donor materials, and good B73breeding excellent inbred lines forreceptor materials.Our plan is to build our hybrid first Mu mediated drought-resistant mutant pool. In thepool as the foundation, has the obvious drought resistance mutations were the phenotype of corn mutant,and carry out the flanks of the insertional locus MU sequence of bioinformatics analysis, forecast ofbiological function flank sequence, and drought resistance mutations associated with characteristics, toclarify the mutations in drought-resistant metabolic pathway, to develop drought-resistant breeding anddrought resistance genes functional genomics research provides a wealth of scientific research material. The main research results are as follows:1. Getting M1generation materials, a total of3010grains. Getting3000tree plantings after seedingM1generation plants.It made2987M2clusters, randomly selected100clusters, a total of37000grainsplanted in indoor potted plant, growed36980trees. After far infrared mutant screening on droughtresistance, and getted19drought resistance of the tree mutant plants.2. The drought resistance to19cases were Mu-TAIL-PCR amplification, contains MuTIR lateralsequence article56. After removing MuTIR among them, the sequence of100-300bp has13articles, thesequence of500-1000bp has28articles, the sequence of300-500bp has15articles.After finishing therepeated sequence sites point, getted10sequences of500-1000bp.3. Article10specific sequences of biological function prediction showed that one and the arabidopsisPPR protein were similar, corn plants and seeds can promote the development of the endosperm, promotechloroplast formed, participate in the chloroplast physiological and biochemical metabolic process, still canreverse the nucleus and control between organelles signal transmission, participate in antioxidant, resist thefunction of adversity. It may be the important reason for the drought in corn..