The Research Of Genetic Diversity Of Leonurus Japonicus Germplasm Resources In Henan Province

Motherwort (Leonurus heterophyllus Houtt.) is an annual or biennial herbaceous plant with medicinalproperties and a worldwide distribution in the Lamiaceae family.Growing in many habitats, especiallyunshaded places, L.Heterophyllus Houtt. can be found in most regions of China. Genetic diversity ofL.Heterophyllus Houtt. has been investigated using RAPD、AFLP and ISSR molecular markers.SSRprimers and the ScoT technique have not been previously employed in the study of L. Heterophyllus Houtt..Using the magnetic beads enriched, SCoT-silver staining techniques and the UPGMA cluster analysismethod, I explored:①the feasibility of SSR primers;②the genetic diversity and phylogenetic relationshipof the66L.Heterophyllus Houtt.samples. The main results are as follows:⑴I have developed several SSR primers. Only preliminary analyses concerning these primers havebeen carried out due to the small number of successfully constructed primers. Futher research in this area isnecessary.⑵I selected ten pairs of SCoT primers from an initial library of36for the analysis, obtaining240clear amplified bands, all of which were polymorphic (100%).①L. Heterophyllus Houtt. appear to behighly polymorphic.②The clustering results show that the geographical distance and phylogeneticrelationship is associated, however, the geographical distance is not the main factors, genetic backgroundfactors play a major role..③Genetic diversity of the samples is dominated by inter-population variations.④Overall, geneticdistance was unobvious positively correlated with geographic distance.