Molecular Detection And Identification Of GmPGIP3 And BvGLP1 Transgenic Wheat With Enhanced Disease Resistance

GmPGIP3 is soybean polygalacturonase-inhibiting protein 3, could inhibition of fungal endo-polygalacturonase acid activity, which weakened pathogen fungi to plant Infection. BvGLP1 is a kind of sugar beet Germin-like protein(GLP), GLP catalytic oxalic acid oxidation to produce hydrogen peroxide, hydrogen peroxide can induce resistance gene expression so that the plants associated with resistance. In this study, the use of genetic engineering technology to build the GmPGIP3 and BvGLP1 genes monocots expression vector pA25-GmPGIP3 and pA20-BvGLP1, by gene gun-mediated and related molecular detection techniques that GmPGIP3 and BvGLP1 into wheat varieties have been promoted Yangmai 18 and Yangmai 19. After several generations of identification and selection, finally picked Enhanced-resistance to Sharp Eyespot, Fusarium Head Blight and wheat root rot new species, the main research work are as follows:1. Access of enhanced-resistance Yangmai 19 to Sharp Eyespot. We characterized the GmPGIP3 transgenic wheat plants in T0 to T4 generations by PCR, Southern blot, RT-PCR, and Q-RT-PCR analyses. We also evaluated the disease resistance in these GmPGIP3 transgenic plants through inoculating R. cerealis. The results show that, GmPGIP3 in different transgenic wheatlines with one to two copies, and the alien gene GmPGIP3 was over-expressed in seven transgenic wheat lines; different transgenic lines disease resistance contrast with Yangmai 19 showed significantly-enhanced resistance to R. cerealis.The ability of disease resistance can be stably inherited, the main agronomic index with no significant differences with receptor material Yangmai 19.2. Access of enhanced-resistance Yangmai 19 to Fusarium Head Blight. We characterized the GmPGIP3 transgenic wheat plants in T0 to T4 generations by PCR, Southern blot, RT-PCR, and Q-RT-PCR analyses. We also evaluated the disease resistance in these GmPGIP3 transgenic plants through inoculating F. graminearum. The results show that, GmPGIP3 in different transgenic wheatlines with one to two copies, and the alien gene GmPGIP3 was over-expressed in seven transgenic wheat lines; different transgenic lines disease resistance contrast with Yangmai 19 showed significantly-enhanced resistance to F. graminearum.The ability of disease resistance can be stably inherited.3. Access of enhanced-resistance Yangmai 18 to wheat root rot. We characterized the GmPGIP3 and BvGLP1 transgenic wheat plants in T0 to T2 generations by PCR, RT-PCR, and Q-RT-PCR analyses. We also evaluated the disease resistance in these GmPGIP3 and BvGLP1 transgenic plants through inoculating Bipolaris sorokiniana. The results show that, the alien gene GmPGIP3 and BvGLP1 was over-expressed in seven transgenic wheat lines; different transgenic lines disease resistance contrast with Yangmai 18 showed significantly-enhanced resistance to Bipolaris sorokiniana.The ability of disease resistance can be stably inherited, the main agronomic index with no significant differences with receptor material Yangmai 18.