Transformation Of OsPTF1into Soybean By Agrobacterium

Soybean is one of the most important oil crops. It is also important grain and feed crops. Soybean also can provide plant proteins. Phosphorus is an essential element of soybean growth and development. Most of the phosphorus in the soil are exist in the form of a solid phosphorus, the plants are difficult to absorb from soil. A long time, people have been through the application of chemical fertilizers to replenish soil phosphorus, although it greatly improved crop yields, it followed a series of ecological and environmental problems, serious fertilizer, environmental degradation, input efficiency decline. Absorb phosphorus in the use of transgenic methods to improve the soybean is a viable option.In order to get the efficient absorption and utilization of phosphorus in soybean genetically modified varieties, efficient phosphorus gene OsPTFl was transferred to soybeans by Agrobacterium-mediated method. Target gene OsPTF1from the rice, insert it with the35S promoter and NOS terminator in pCAMBIA1300, and then inserted to highly plant efficient expression vector pTF101.1together with the promoter and terminator was cut. Then transferred it to Agrobacterium tumefaciens EH105for project Agrobacterium. Ultimately, we get16resistant plants through PPT screened. After the initial detection of PCR molecular three transgenic plants integrated a OsPTFl exogenous gene.Screening soybean genotypes on the part of the Huang-Huai-Hai region in order to improve soybean transformation efficiency. Genetic transformation plant expression vector agrobacterium tumefaciens contained p1300-221(containing GUS), alternative soybean genotypes tested first pre-culture time and receptor tissues-day cotyledon node, through the co-cultured GUS staining, identification of the conversion efficiency of all varieties, the appropriate choice as the transformation of the genotype of the receptor. Soybean varieties Zheng59, Yudou22, Shang dou1099, the Zheng EH04023, the Zheng EH04063and Jack, inoculated on MS medium, the results indicate that after inoculation Zheng59and some other varieties, the growth of cotyledon cracking phenomenon is serious. In addition, the induction of callus is also different due to genotype differences. After infection results showed Shang dou1099and Jack infection effect better than others, after culture showed two species callus growth better too. Select the infection site, we found that infection of the cotyledon node effect is better than the embryo tip infection effect. The way of emergence, we compare the common shoot elongation and rooting medium induced the emergence and grafting callus directly to ordinary soybeans for the emergence of stock, grafting time is short, strong seedlings, the final selection of the manner emergence is grafting.