The Expression Of Bax，Bcl-2, Caspase-3and Caspase-9Exposure Zearalenone Mycotoxicosis Of Ovaries In SD Rats

Objective: To determine the influence of Zearalenone (ZEA) on the expression of Bax,Bcl-2, Caspase-3and Caspase-9in the ovaries of SD rats. Immunohistochemical ultrasensitive SP method has been used to examine the expression of Bax, Bcl-2,Caspase-3andCaspase-9in the control rats and injected with ZEA oil rats in the study.Method:30SD rats at Ten-week-old were randomly divided into two groups andsimultaneous feeding. Test group (n=15): treat with0.5mL5mg/kg i.p. of ZEA.Controlgroup (n=15): treat with equal saline. Euthanized at3,6,12,24and48h after treatment inorder to determine the expression of Bax, Bcl-2, Caspase-3and Caspase-9at different times.Result:1. Copy of the pathology model on rats zearalenone intoxication Rats mainlyshow following symptoms: labia swelling, spirit weidun, loss of appetite, etc. Pathologicalchanges are expressed as follows: liver congestion swelling and texture crisp,lymphadenopathy, bleeding points on thymus, kidney congestion, Lung bleeding and becomered, tubal uterine edema, ovarian hyperemia. histopathological change：Control group werefound degeneration in liver cell and dilation of lobule central vein which were full of redblood cells; degeneration in the epithelial cells and luminal stenosis of the proximal tubules;plenty of megacells and hemosiderins in dilative splenic sinus; angiotelectasis in smallintestinal inherent layer and submucosa, tons of red blood cells in capillary vessel and visiblemacrophages infiltrates in tissue space; follicles in ovarian tissue were atretic.apotosis cellwere found in granular layer of ovarian follicle. By MG&P stain and TUNEL method, it isconfirmed that ZEA can result in apotosis of granular layer cell of ovarian follicle.2. The expression of Bcl-2and Bax the expression of Bcl-2and Bax in both experimentgroups and control groups and presented dynamic changes as time went on. Bax expressionshowed a significant increase compared to control groups (P﹤0.05) in3h,6h,12h andshowed steady decline at24h and48h. The expression of Bcl-2showed no statisticalsignificance compared to control groups（P﹥0.05）.3. The expression of Caspase-3and Caspase-9Caspase-3expression showed asignificant increase compared to control groups (P﹤0.05) in3h,6h,12h,24h,48h. Theexpression of Caspase-9showed a significant increase compared to control groups (P﹤0.05) in3h,6h,12h and showed no statistical significance compared to control groups at24h and48h（P﹥0.05）..Conclusion: Zearalenone mycotoxicosis can result in ovarian follicles damage andgranulosa cell apotosis in rats. Bax,Caspase-3and Caspase-9play important role in apoptosisof granulosa cells in the rats with zearalenone mycotoxicosis and the influence of bcl-2is notobvious.