Study On The Pattern Of Variation Of The Main Active Ingredients In Ganoderma Lucidum With Different Sources

Based on the main varieties of G.lucidum with different sources, the main active ingredients,chromatographic fingerprint of triterpenoid constituents and the antioxidant activity of spore powder ofG.lucidum were studied which aimed to reveal the genetic variation of different sources of G.lucidum,the oxidative stability of spore powder and to provide a scientific basis for the cultivars breeding andquality control of G.lucidum..1. The content of total polysaccharide, triterpenoid and protein were determined by UVspectrophotometry. Ergosterol was analyzed by HPLC. The fourth with different active constituentsfrom the different sources of G.lucidum have been detected. The result revealled that the totalpolysaccharide is closely related to the cultivars and culture substrates, the content of polysaccharidewhich cutivated by basswood was siginificantly higher than which cutivated by bag. The content oftriterpenoid is8.15~9.64mg·g~-1and there was no siginificant differences between different sources of G.lucidum.The content of protein is closely related to the cultivars but has a low relativity with culturesubstrates.. Ergosterol has a siginificant difference in the fruit bodies of G. lucidum and the content of G.lucidum is higher than G.sinense.It will increase the contents of total polysaccharide, triterpenoid andergosterol by breeding excellent cultivars and selecting superior culture substrates.2. Using RP-HPLC method, the chromatographic fingerprint of triterpenoid constituents HPLCfingerprints were established. The chromatographic conditions were as follows: using a gradient elutionwith acetonitrile-water containing0.5%ethylic acid as the mobile phases. The detection wave lengthwas250nm, the flow rate was0.8mL·min-1and the temperature of column was30℃.Established thefingerprints of ten batches of G.lucidum from different producing areas and using Similarity EvaluationSystem for Chromat-ographic Fingerprint of TCM2004to assess the ten samples, and qualityassessments were analyzed by smilarity. The result showed that the HPLC fingerprints of ten batches ofG.lucidum consists of18peaks, and the range of similarity is between0.449-0.735. The diversity islarge between the10varietal G.lucidum from different sources. In order to evaluate the quality ofG.lucidum comprehensively, the only one index of triterpenoid is not precised. 3.The schaal oven method was used to speed up the oxidization of the spore powder of G.lucidum.In order to detect the oxidative stability of spore powder different anti-oxidants were added tothe spore powder and the acid values(av),peroxide values(pov) were determined.The result showed thatoxidative lipid peroxidation occurred during the time passed and an increase of av,pov along with thetime passed too, the sample which added with tert-butylhydroquinone (TBHQ) improved the shelf lifeby8months.The shelf life of the spore powder of G.lucidum in ZHE JIANG Province is16months andit has the ability of antioxidation,strong stability and easy to store. It can provide scientific basis for theshelf life of spore powder of G.lucidum through the study of oxidative stability.