| High latitude cold area of arid environments lack appropriate ornamental plant resources, Daqing grassland Sandy arid habitats of perennial ornamental flower resources, garden nursery in urgent need of appropriate local climate environment green plant resources. In order to watch good Daqing sand soil arid habitat distribution of flower resources of Phlomis tuberosa L as tested materials, discusses the optimization of seed propagation technology and drought resistance. In the arid environment of the landscape to provide a scientific basis.At the Phlomis tuberosa L infructescences in different parts of the seed, the seed will be divided into three parts of experiment, seed germination and temperature, illumination, and the provenance relation matrix. And PEG (5%,10%,15%,20%and25%) and NaHCO3, NaaCO3simulated drought stress, and mixed salt (0.02,0.04,0.06and0.14mol/L) on its germination, from the perspective of holographic biology collection of high quality seed and its optimized seed position, of high quality seedling propagation technology process; and to Phlomis tuberosa seedlings with different concentration of PEG (10%,20%and30%) simulated drought stress on Seedling of artificial control of water drought stress research, evaluation of the drought resistance. The results show that:In1, Phlomis tuberosa seeds thousand-grain weight for3.393g; germination temperature of20to25℃, the germination rate is27.78%～46.00%; for the light sensitivity of seeds, in a lightless condition to germination; the most suitable cultivation substrates for Peat soil:sand:perlite:vermiculite=3:5:1:1, the germination rate reaches69.00%; after artificial management, breed, can raise the Phlomis tuberosa seeds germination rate of37%～45%. The upper1/3section seed the indicators were higher than in the lower part of the seed, as the best take part.In2, PEG10%～15%, seed treatment, germination rate is the highest, reaching52.33%～52.11%; the upper seeds in PEG15%treatment germination rate was82.67%, a water treatment increased by36.67%. With the same concentration of PEG processing central seed increased22.67%, a lower seed improves60%.0.06mo1/L Na2CO3and mixed salt and0.14mol/L NaHCO3inhibited seed germination, the top seed germination rate drop respectively34.17%,36.33%and31.67%. The germination rate of average values were decreased by17.27%,17.34%and11.00%. Phlomis tuberosa seeds during germination drought tolerance is not resistant to saline alkali stress.In3, Phlomis tuberosa seedlings in the PEG10%,20%and30%respectively in12h, under the stress of8h and4h after the start of a gradual loss of ornamental. In the PEG30%stress in20h arrive at the4level of wilting, eventually lose their ornamental characteristics. While the seedling in water control30d, plant wilting dry, lose their ornamental characteristics;35d, above ground plant parts of death, enter a state of dormancy;35d after rewatering, plant to send out new leaves, growth again. Effects of drought stress on the Phlomis tuberosa and physiological index of effect. Net photosynthetic rate, transpiration rate, water use efficiency, stomatal conductance, leaf water content, the chlorophyll content was negatively correlated with stress intensity, MDA content and electrical conductivity and stress strength correlated positively, can be used as the root of Phlomis umbrosa Drought Adaptability Evaluation factor. Unit leaf area dry matter, soluble protein content, intercellular CO2concentration and SOD activity can only partly reflect plant adaptability, can be used as reference. |
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