De Novo Assembly And Analysis Of Teosinte Seedling Transcriptome Defined By RNA-Seq

The domestication of maize begins at the dawn of human agriculture,about10,000years ago, and the arguing of the ancestor of maize has last for many years. These years, re-searchers studied the Chromosomal number and morphology, choroplast, ribosomal, isozyme and molecular genetics evidence, and demonstrated that one of teosinte, Z. m. ssp. parvig-lumis is the immediate ancestor of maize. Our research did the De novo assembly of the teosinte transcriptome with RNA-Seq technology and studied the transcipts by calculating the expressing level, comparing with B73and other three monocots’ transcriptome of pro-teome, functional annotating through GO, KEGG, KOG, UniProt database.Our research sampled6species of teosinte and sequenced the cDNA library by solexa method. Firstly, we controled the quality of obtained sequeces, and kept77%eventually. After ensuring the high quality of the sequences, we did the De novo assembly using Trinity and TGICL tools and got58,147teosinte transcitps whose average length is up to1335bp, which is shorter than B73’s. All the following studies are based on these tanscripts. We en-sured the accuracy of the assembly by RT-PCR validation. Then we compared the teosinte transcriptome with B73, sorghum, bra and rice, found some sequences with high-similarity. After that, the expression value was calculated by RPKM method. In the process of anno-tation, we used data from some common annotation database, of which UniProt has most widely annotation.Our study on teosinte transcriptome validates the close relationship between teosinte and maize, supplys the sequence information for future research of teosinte, and can also help to discover the key genes responsible for the notable mophology differences between teosinte and maize. And the method described in this paper will supply meaning information to the future study of plants without reference genome sequences.