The Study Of Mechanism On Rat Obesity-induced Intestinal Injury

Obesity has become a serious threat to human health worldwide the disease. Recently reported that rats fed high-fat foods increased intestinal permeability, and produces the metabolism of endotoxemia. Endotoxin may be associated with high-fat foods cause inflammation, which inflammatory mediators or bacterial toxins released into the blood ROS was activation of ROS can cause lipid peroxidation, as a second messenger regulation of proinflammatory cytokines and chemokines, which destruction of the intestinal barrier, triggering apoptosis and necrosis. In view of obesity developing chronic inflammation, and damage the intestinal mucosal barrier, In this experiment, the establishment of the rat nutrition diet-induced obesity model, and then take the ileum and colon tissue using the QPCR method analysis of NF-κB (Nuelear faetor kappa-light-chain-the enhancer of aetivated cells B), Stat3(Signal transducers and activatorsof transcription3) and Survivin gene expression levels. Research obese rats and obesity resistant rats, to investigate the damage mechanism of obesity, inflammation of the intestinal mucosal barrier.The results:1. In the high-fat diet-induced rat ileum of NF-κB expression was significantly up-regulated (P<0.05)., show the description of the high-fat diet induced oxidative stress, causing inflammation of the ileum2. In the ileum and colon, the Stat3expression of the negative control group and the obesity-resistant group is higher than the expression of the obesity model group, and significant differences in the ileum (P<0.05).3. In the ileum and colon, obesity model group and obesity resistance group Survivin expression were compared with negative control group of high expression levels and obesity resistance group expressed slightly higher than the obesity model group, but the difference was not significant. In the colon, obesity resistance group and the obese group Survivin expression was significantly higher than the negative control group (P<0.05).The conclusion:1. The high-fat diet induced oxidative stress, causing intestinal inflammation,and the degree of inflammation of the intestinal of the obese rats is higher than the obesity-resistant rats; in the ileum and colon of the obesity-resistant rats, high-fat diet-induced intestinal inflammatory factor expression differences in the organization..2. Stat3and Survivin gene expression in the gut into a positive correlation.