Effects Of The AadA Transplastomic Tobacco On Microorganism And Enzymes In Soil

In this paper, the effects of aadA transplastomic tobacco (TA) on soil microbialcommunities were explored by dilution plating, Biolog Ecoplate and DGGE methods,together with analysis of soil enzymes activities during seedling stage, vegetating stage,flowering stage and senescing stage. The main contents are as follows:1. The population numbers of microorganism were investigated by dilution platingmethod. The colony units were the least at seedling stage, a little increase at vegetativestage and peaked at flowering stage, then begun to decrease at senescing stage. Therewas no significant difference on the population numbers of microorganism betweenaadA transplastomic tobacco and WT at the corresponding stages.2. The functional diversity of rhizosphere microorganisms was detected by BiologEcoplate method. The similar trends of AWCD were observed for both aadAtransplastomic and WT plants. The Shannon, Simpson, Mcintosh diversity indexes andPCA were analyzed. The results showed that the aadA transplastomic tobacco had noeffect on microbial community during two years of growth.3. DGGE analysis indicated that the banding patterns, bacterial communitydiversity and PCA were similar for both ecotypes. The aadA transplastomic tobacco hadno significant effects on microbial community diversity.4. Soil enzyme activities were tested. The results showed that the activities of sixsoil enzymes were the least at seedling stage, increased at vegetative stage, peaked atflowering stage, and then decreased at senescing stage. There were no significantdifference between aadA transplastomic tobacco and WT at the corresponding stages.