Effects Of The Fad8Transplastomic Tobacco On Microorganism Populations In Rhizospheric Soil

In the present study, by using dilution plating, Biolog Ecoplate, DGGE methodsand soil enzymes activities analysis, we comprehensively investigate the effects of fad8transplastomic tobacco and ecotypes on microbial number, community structure,functional diversity and soil enzyme activity in the seedling stage, vegetating stage,flowering stage and senescing stage. The main contents are as follows:1. In the whole growth period of tobacco plants, rhizosphere microorganisms thatcan be cultured were less in seedling stage, and began to increase quickly in vegetativestage, to maximum in flowering stage, whereas it has begun to decrease in senescingstage. Compared to WT, the fad8transplastomic tobacco had no distinct effect onmicroorganism population. However, significant difference was discovered betweendifferent cultivars.2. BIOLOG results showed that the fad8transplastomic and control tobacco weresimilar in AWCD; The Shannon, Simpson, Mcintosh diversity indexes and PCAanalysis showed that the difference between fad8transplastomic and WT plants was notsignificant in all periods, whereas the difference between cultivars were apparent in theseedling and senescing stage.3. DGGE analysis showed that in the whole growth period of tobacco, bacterialcommunity diversity between fad8transplastomic and WT plants had a slight difference,but the statistic analysis showed no significant difference. The similar result was alsoobserved for different cultivars.4. In the whole growth period of tobacco plants, the variation trends in activities ofsix soil enzymes including urease, protease, catalase, polyphenol oxidase, invertase andcellulase were similar. Soil enzymes activities were not affected by both fad8transplastomic tobacco and different cultivars.