QTL Analysis Of Appearance Quality Related Traits In Rice(Oryza Sativa L.)

Appearance quality, one of primary targets of rice breeding, includes grain shape and chalkiness.They affect not only rice yield, but also the quality and the market value. In the present study, wecarried out QTL mapping of some traits including grain shape, chalkiness after constructing a ricegenetic linkage map.1. A rice F₂population consisting192lines which derived from a cross between YZD andⅡ-32Bwas utilized for constructing a rice linkage map. The linkage map including94simple sequence repeat（SSR） markers covered1351.6cM on total12chromosomes, with an average interval length of13.25cM.2. QTL analysis was performed using the software WinQTL Cartographer2.5.16QTLs whichhave significant additive effects were indentified, only qGL3was found in two years. The main-effectQTLs, qGL3, qGL7, qPGWC6explained41.89%,26.37%,19.12%of the phenotypic variance,respectively.3. Two rice BC₂F₂populations were used for verifying qPGWC6, which were planted ingreenhouse and field. A QTL was mapped on the same location as qPGWC6under greenhousecondition.which is grown in greenhouse, explained40.56%of phenotypic variance.4. There is microsatellite polymorohism in wx gene at5′-untranslated region, and YZD andⅡ-32B had （CT）18and （CT）11. There is a SNP site （AGT/GTATA）, at the putative leader intron5′splice site, and YZD and Ⅱ-32B were AGGTATA and AGTTATA, respectively.