Cell Culturing And Chromosome Karyotyping Of Antlerogenic Periosteum Of Sika Deer (Cervus Nippon)

Sika deer (Cervus Nippon) is an important special economic animal in China which is famous forits main economic product---deer antler. Deer antler，the main secondary sex characteristic of buck，shed periodically and can regenerate rapidly. Deer antler can grow with a speed of1-3cm per day duringrapid-growing, while with the cell differentiation speed30times faster than cancer cell. All thesecharacteristic make deer antler become a special biomedical model.Antlerogenic periosteum of sika deeris the stem cell to form the deer antler， study on these cells can help us to understand the mechanism offormation and regeneration of deer antler， while the in vitro cell culture of the periosteum will providethe foundation of the further study such as amputative limb regeneration and cell growth regulation.This paper is focused on optimization of antlerogenic periosteum stem cell culture system and thestability of chromosome of antlerogenic periosteum stem cell. The methods and conclusion are asfollows.This experiment studied the antlerogenic periosteum cell of sika deer from Institute of SpecialEconomics Animal and Plant Sciences, discussed the optimization of primary culture, secondary culture,cryopreservation and recovery. The result shows that the stem cell can remain full vitality, the timeinterval between secondary culture is2-3d, the cells had vigorous growth after cryopreservation insubstrate added with10%DMSO. In order to study the stability of the cell hereditary, the chromosomeof antlerogenic periosteum cell after15times subculture was also studied. The paper discussed thecondition of chromosomal preparation of antlerogenic periosteum cell. The results shows that67of80cells have chromosome number as2n=66, which take84%in total, confirmed that the antlerogenicperiosteum cell still have steady biological characteristics after in vitro culture.