Screening Of High-temperature Cellulose-degrading Bacteria And Nitrogen-fixing Bacteria And Composite Agents Cultivated In The Grass Rot Fungi

Efficiently using cellulose is one of the focus of new energy and sustainable development, enzymes bacteria technology is an effective method of the current pollution-free handling and resource utilization of cellulose. This paper summarize the development course of enzymes bacteria and enzymes bacteria technology in the industrial and agricultural production, and overview related research progress at home and abroad. On this basis, the cultivation of grass rot fungus is not completly decomposite, decomposition cycle long, labor-intensive, high cost of production and pest control problems and other issues, mainly in aerobic thermophilic cellulase activity, efficient strains of nitrogen-fixing ability and strain identification, and preparation of efficient complex microbial strains agent, making compost fast composting, compost time, at the same time,improve the quality of compost. Different composting conditions for aerobic composting; and enzymes bacteria products are used in the cultivation of the grass rot production, making the cellulose to get a good sound processing, grass rot feed production cycle reduced, quality increased, to reach another fast, and good target for the production of grass rot fungus.This experiment was isolated cellulose decomposing bacteria and nitrogen-fixing bacteria, and screened strain combination vaccination vaccination agents for composting, the composting of grass rot. Draw the following main conclusions:1.Different sources of grass rot materials, enzymes, bacteria products, screened three cellulose-degrading bacteria to the cultivation of different crops to soil screening to a nitrogen-fixing bacteria;2. Determination of the genetic background of physiological and biochemical characteristics and molecular biology of the two bacteria were identified by the morphological characteristics of the strains and cultural characteristics, actinomycetes, Streptomyces, fungi, small spore Rhizopus genus;3. Determine the production curve of the four bacteria, the ratio of its fermentation performance of the compost in the composite fermentation strains, and screening to the optimal compound ratio:actinomycetes:fungi:bacteria:Azotobacter=2:2:1:1; examine the water content in the composting process, composting size, ambient temperature and ventilation of fermentation, screened compost fermentation conditions:moisture control between60%-65%, scale suitable for composting large-scale fermentation, the ambient temperature is relatively high, the ventilation for every3d turning once.4. Production obtained by the proportion of optimal species complex, the optimum fermentation conditions, the activity of bacteria generation of the original species.