The Research On Genetic Evolution Relationship Of Quercus. Mongolica And Quercus. Wutaishanica

The Quercus L.plant is one of the broad-leaved forest species constructing,mainly in subtropical and temperate regions,plays an important role in the planet’s ecosystems.Quercus L.is the largest genus of Fagaceae,about450kinds,China is one of the Quercus L.species distribution center,the country were distributed,with many endemic species in China.Quercus L.wide range,but has always existed in the Quercus L. species within the morphological differences between species form overlapping, resulting in unclea classification, scholars are most concerned about is the Q.mongolica and the Q.wutaishanica debate Q.mongolica and Q.wulaishanica morphological less obvious, since the establishment of Q. wutaishanica the academi community is very concerned about the phylogenetic relationship between Q.mongolica and Q.wutaishanica.In this study,600from25wild populations of Q.mongolica and Q.wutaishanica material was analyzed from three aspects of morphology, nuclear loci and trnH-psbA gene sequencing,a white Q.aliena groups as outgroup,and genetic evolutionary relationship,including the following areas.1. morphological research:Setting Q.mongolica and the Q.wutaishanica9leaf morphologytraits unweighted pair group method with arithmetic mean cluster analysis results showed that Q.mongolica and Q.wutaishanica is divided into two:one including northeastern Heilongjiang, Jilin and eastern Inner Mongolia and Liaoning part of the region,a total of11groups; another part of northwest Gansu, Shaanxi, Shanxi, central Inner Mongolia and southern Liaoning region,a total of14groups. Principal components analysis reflect the results of8morphological features reveal the phylogenetic relationship and taxonomic studies of very important significance between species of Q. mongolica and Q.wutaishanic.2. Microsatellite loci research:Using9nuclear DNA microsatellite loci in600samples of25Q.mongolica and Q.wutaishanica group and Q.aliena a group were analyzed. Cluster analysis of the genetic structure of populations,26group were significantly composed of two major components, the group of Northeast and Northwest groups obviously divisive northwest groups and Q.aliena groups gathered together.3. trnH-psbA gene sequencing research:Chloroplast trnH-psbA gene sequencing on the Q.mongolica and the Q.wutaishanica and Q.aliena624samples from26groups, downloaded from Genebank Q.mongolica trnH-psbA gene sequences outsidegroups to a common root analysis,a total of17kinds of haplotype. The results showed that. Q.mongolica and Q.wutaishanica groups from the two missing ancestors type,an ancestor mv4of the type1haplotypes mainly in the northeast group, another ancestor mvl of the type4haplotype in northwest groups, the haplotype their own differentiation, between have cross,in southern Liaoning, central Inner Mongolia and other placest produce exchange.Integrated three aspects of data analysis results,the paper conclude that the Q.mongolica and the Q.wutaishanicais differentiation species,they come from different ancestors groups. Q.mongolica as the main distribution area in the part of Heilongjiang,Jilin and eastern Inner Mongolia,Q.wutaishanicais as the main distribution area in the part of Shaanxi,Shanxi,the existence of gene flow in the distribution of overlapping regions.