Promotion Of Plant Growth And The Development Of Microbial Fertilizer By Pseudomonas Aurantiaca JD37

Our country is the large agricultural nation, in order to increase cropyields and the extensive use of chemical fertilizer has become acommon, but this will on the ecological environment, and even humanhealth and the environment a great deal of harm. Urgently seeking anenvironmentally friendly solution, not only can increase crop production,without causing environmental hazards. Plant growth-promotingrhizobacteria(PGPR), which can promote plant growth, prevent disease,increase crop yields. At present, the use of PGPR strain developmentand application of microbial fertilizers has become a hot topic. Ourisolated one strain from the rhizosphere soil which named Pseudomonasaurantiaca JD37, it is a PGPR strain. The study found that the JD37strainhave broad-spectrum antagonism to plant pathogens, can significantlypromote the growth of the monocot Gramineae plant corn and wheat,to prevent plant disease. The JD37strain could produce siderophoreand dissolve phosphorus. It can induce the plant to build systemicresistance. So the JD37strain have the potential of the development ofmicrobial fertilizer.In this experiment, we organized a series of tests including culture onplates in laboratory,greenhouse tests and field tests by using manyindexes such as tomato seed germination rate, root length, seedlingheight, fresh weight and dry weight to study the growth-promoting ofJD37strain on tomato. TLC chromogenic method and the ACC usingablitiy test to analysis the growth effect mechanism of JD37strains. Ourstudies show that deal with bacterial can significantly promote tomatoseedling root growth. Indicating that strain JD37promoting effects ofdifferent types of plants there are differences. The dicotyledonous,solanaceae fruits and vegetables plant of tomato seeds wereinoculated with JD37strain, the most appropriate method is suspension. In the pot growth-promoting experiments of tomato by JD37strain, wemeasured several index containing average root length, averageseedling height, average fresh weight and average dry weight.Compare our findings to the control is respectively increased37.41%,18.20%,69.62%,70.97%. Suggests that the tomato plant grows the mostsignificant effect of bacterial density is107cfu/mL of JD37strain. TLCchromogenic method and the ACC using ablitiy test shows that theJD37strains can secrete the plant growth hormone of IAA and can beinduced ACC deaminase.The interaction between PGPR strains and AM fungi of the preliminaryresearch results show that the plant growth-promoting effect byco-inoculation are greater than the separate inoculation. A potexperiment shows that co-inoculated of JD37strains and AM fungisynergistically increased plant growth compared with singly inoculatedplants. Densities of JD37strain in the rhizosphere of30-day-old plantsinoculated with JD37strain and AM fungi (1.2×106cfu/g) weresignificantly higher than those shown by JD37strain(2.3×104cfu/g), theindicating that AM fungi beneficial to the JD37strains growth,reproduction and increased the role of the JD37strain, and thus moreeffective in promoting the growth of plants. At the same time,mycorrhizal colonization after30days of culturewas increased by8.95%in plants co-inoculated with the JD37strain and AM fungi.Pseudomonas aurantiaca JD37strain had the strong role of biologicalcontrol and plant growth promotion. So JD37strains have developmentfor the potential of the microbial fertilizers. In the whole developmentprocess, from the carrier and the protective agent to research JD37preparation of microbial fertilizer. Through research of adsorption,preservative period and the release rate of JD37strain.In screeningexperiment, to determine JD37microbial fertilizers dry power wereselected talc as carrier material and0.1%CMC as protective agent. To investigate the effect of JD37microbial fertilizers, use of its water solutionto inoculate the tomato, the date show that the bacterial populationwas maintained about106cfu/g in the rhizosphere soil and105cfu/g inthe internal roots of tomato and ignificantly promoted growth of plantseedling. In the six months of preservation period, the number ofbacterial effectively maintain in7.68×1010cfu/g.