The Physiological Activity And Protein Antibacterial Activity Of Tartary Buckwheat During Germination

The purpose of this paper was to study the physiological activityand protein antibacterial activity of tartary buckwheat duringgermination. The results were showed in Chapter Two to Chapter Five.In Chapter Two, influence of bud force index(germination force,germination percentage, bud length and bud weight) of tartarybuckwheat with different germination condition were studied. Theexperiment of light treatment showed that the bud force index oftartary buckwheat had declined respectively with natural lighttreatment, dark processing, the dark and light treatment and all thelight treatment. The bud force index of all the light treatment showedsignificant differences with each other(P=0.01). The experiment oftemperature treatment showed that germination force, germinationpercentage and bud length had declined accordingly with naturaltemperature treatment,37℃+30℃treatment,30℃treatment and37℃treatment, significant level at P=0.05.The experiment showed that thevariable temperature can help buckwheat sprout better. Theexperiment of magnetic field intensity showed that with the scope ofthe magnetic field intensity increased, the bud force index ofbuckwheat germination had upward trend in0T-0.6T. It’s the maximumof germination percentage, bud length and bud weight as themagnetie field took5minutes, while germination percentage was85%,bud length was9.4cm, bud weight was0.137g. The metal processingexperiment showed that the buckwheat germination had significantinhibition when the concentration of Aluminum ion reached to1mmol/L.It can improve the germination force of buckwheat that theconcentration of Copper ion choosed0.4mg/L. The research revealedthat low concentration of Potassium permanganate can promotegermination and growth, high concentration had the inhibition. It can reach to maximum of bud force index when the concentration ofPotassium permanganate is200mg/L. Germination force was80%,germination percentage was100%, bud length was10.2cm and budweight was0.140g.In Chapter Three, the research and application of GABA during thetartary buckwheat germination was studied. This paper selects the twodetermination methods, one is Berthelot colorimetric method and theother is OPA pre-column deriverization and high performance liquidchromatography. The former method is applicable to GABA qualitativeanalysis, and the latter in GABA quantitative analysis. Chromatographicworking conditions is that using C18mobile phase,50NaAC (pH=6.8):methanol: THF=65:34:1, Detector: FLD: Ex:338nm, Em:425nm,Velocity:1.0mL/min. The experiment indicated that as days increased with thegermination, GABA content increased and reached the maximum atthe forth day,26.96mg/100g. The change tendency of enzyme activityof GAD was as the same as the GABA, so it is close relationship betweenGABA and GAD in germination. Conditions to improve content of GABAin buckwheat germination were optimized by Densign Expert throughCenter composite design: magnetic field intensity0.265T, ultravioleteffect time10.85min, concentration of copper ion0.0046mg/L. The richin GABA buckwheat beverage formulas was buckwheat germination4th day10g/L, sugar30g/L, honey is0.8g/L. The content of buckwheatbeverage was18.57mg/100g.In Chapter Four, the flavonoids of buckwheat germination wasstudied. The paper determined the best extraction conditions, thatethanol concentration70%, extraction temperature60℃, liquidmaterials than1:50, extracting time480min. The research showed thatthe flavonoids increased along with the extension time of buckwheatgermination, and at the7th day achieved the maximum. The metal ionexperimental showed that processing samples and contrast samples had significant difference. Low concentration of metal ion can improveflavonoids content of buckwheat, and high levels can inhibit thegeneration of buckwheat flavonoids. The most obvious role is KMnO4.Set up a method of isoflavones detected by HPLC, thechromatographic working conditions for chromatographic column:Zorbax SB-using C18, velocity1mL/min, column temperature30℃,detected wavelength260nm, into the sample weight10μ L. TheExperiments showed that glycitin had been produced a small number,21.13ug/g at the4th day.Chapter Five researched on the antibacterial antivity of buckwheatprotein. With the increase of germination days, buckwheat total proteincontent indeclining and trend is slowing. As raw material, buckwheatprotein extracted by saturated ammonium sulfate and purifying theprotein through DEAE-Sepharose Fast Flow, and got BP1, BP2and BP3.The antibacterial experiments showed that the rat typhoid Salmonellatyphi antibacterial circle was1.0mm when the concentration of BP3was25%, the Strawberry Botryis cinerea bacteriostasis laps was0.8mmwhen the concentration of BP3was50%, the Strawberry Botryis cinereabacteriostasis laps was2mm when the concentration of BP3was100%.BP3is low or no activity on Escherichia coli, Staphylococcusaureus, Bacillus subfilis, Penicillium, and yeast fungi. Sodium dodecylsulfate–polyacrylamide gel electrophoresis (SDS-PAGE) confirmed thehomogeneity of BP3and its molecular mass was about66.2kDa.