Studies On The Control Technique Of Corn Stalk Rot

Corn stalk rot is a main disease of maize in agricultural production.In recent years,owingto the the extensive planting of single varieties，agricultural tillage system and the climate, thedisease is spread to many maize production area and becoming more and more serious. Atpresent, there is little study about the disease both at home and abroad, especially having nouseful pesticide to it.The test lists two better fungicides to control corn Stalk Rot Pathogenson the basis of virulence indoor tests. By a field experiment, the test aims at studying thespatial distribution of corn stalk rot, the plant chlorophyll content, malondialdehyded andeffect on yield; the impact of pharmaceutical corn plant chlorophyll content, root activity,photosynthetic enzymes, MDA, the protection of enzyme activity and soluble protein withCorn stalk rot incidence level conditions was studied, Provide a theoretical basis for yieldloss prediction and scientific control of production of the disease.1. The biological activities of seven different bactericides comprising of single-doseand mix-tures were tested against the Pythium aphanidermatum and the Fusariummoniliforme by toxic media method in lab. The results indicated that both tebuconazole andcarbendazim showed strong toxicity against Pythium aphanidermatum, and EC50for mycelialgrowth was2.01mg/L and2.03mg/L respectively, the toxicity of hymexazol andpropiconazole against Pythium aphanidermatum was also strong. Tebuconazole andcarbendazim showed strong toxicity against Fusarium graminearum, and EC50for mycelialgrowth was0.423mg/L and1.37mg/L respectively, besides, the toxicity of difenoconazoleand propiconazole against P Fusarium graminearum, was also strong.2. Field trials of quantitative artificial inoculation about2years were conducted toascertain the effect of every corn’s Corn Stalk Rot, growth vigor, chlorophyll content andphotosynthetic characteristic of leaves at different positions, root activity and grain yield at5～6-leaf stage, large bell stage, filling stage and wax ripeness stage. The results showed thatin5～6-leaf stage，the corn stalk rot has infected the plant; In large bell stage and earlierfilling stage, the growth vigor, chlorophyll content,soluble protein and root activity of theplants were not significantly different with the healthy. But chlorophyll content, solubleprotein and the root activity were lower than healthy plants when corn stalk rot infectfs again at the end of filling stage and happened at wax ripeness stage. We can conclude that thegrowth vigor, root activity and yield components, such as grainweight per ear, mass per1000,ear length, were significantly lower than the healthy.3. Field trials of quantitative artificial inoculation about2years were conducted tostudy the control effect of corn stalk rot with different concentration of carbendazim andtebuconazole and its effect on photosynthetic enzyme,root activity,kinds of protectiveenzyme activities, MDA activity and the yield. The results showed that,with concentrationsincreasing between different treatments of60g/L tebuconazole FS, strengthen the cornprotection, improve the control effect, promoting the effect of yield increase compared withthe control. The protective effect,control effect and the yield reach the highest level, thecontrol effect reached73.90%, the yield increased40.35%compared with the control, whenthe concentration was20mL/10kg seeds; The protective effect,control effect and the yieldreach the highest level, the control effect reached74.15%, the yield increased39.47%compared with the control, When the chemical/seed ratio is1:100of80%carbendazim WP.Confirmed the feasibility of the seed treatment control the corn stalk rot,but control efficiencyaffected by environment.