Localization Of ERα、ERβ、PR And GHR And Their Differential MRNA Expression In Mammary Gland Of Jining Gray Goat During Postnatal Development

Jining Gray Goat is a high prosperity goat breed and famous for its early maturity,high reproductionrate and good quality of kid which located in the southwest region of Shan Dong province.The puberty ofewes begin at2months of age and sexual matyrity at3to4months of age,the average lambing rate is up to283percent(Cai quan,2008).The objective of the experiment was to study the level of ER、PR and GHRproteins with The SP immunohistochemical staining, the abundance of ERαmRNA、PRmRNA andGHRmRNA by the real time fouorescence quantitative PCR,and the changes of number and distribution ofmast cells and eosinophils in mammary gland during postnatal development in Jining Gray Goat.Resultsshowed that:ERα-immunoprotective staining was malinly localized in the nucleus of blood vesselsendothelial cells, stromal cells and ductal epithelial cells of mammary gland,occasionally in cytoplasmic,ERβ mostly stained in nucleus and cytoplasmic at the same time.The average optical dencity was graduallyincreased from birth day to sexual maturity, and it was significantly higher in sexual maturity than birth dayand puberty (P<0.01),which was almost flat with180days (P>0.05). The results of RT-PCR showed thatthe abundance of ERαmRNA was gradually increased from birth day to sexual maturity, and it wassignificantly higher in sexual maturity than birth day and puberty (P<0.01),there was a slightly decreasefrom sexual maturity to180days. In puberty, the average optical dencity of ERβ was the least,while theabundance of ERβmRNA was the hightest. PR had similar variations with ERα, PR-ip substance alsofound in alveolar epithelial cells in180days. The average optical dencity was gradually increased frombirth day to sexual maturity, and it was significantly higher in sexual maturity than birth day and puberty(P<0.01),which was almost flat with180days (P>0.05).While the abundance of PR mRNA from birth dayto sexual maturity was lower and there was no significant difference during these groups. In180days,itwas significantly higher than the other three groups (P<0.01). GHR–ip staining was malinly localized inblood vessels endothelial cells, stromal cells and ductal epithelial cells of mammary gland and mainlydistributed in the stromal cells at puberty.The average optical dencity of GHR and the abundance of GHRmRNA had the same changes. At puberty and sexual maturity,they were higher than ERα and ERβ.The number of mast cells is the lest in birth day, while a surge in30days,and it increased from60days to120days. The mast cells whose cytoplasm stained dark blue purple were mainly distritubed in intralobularconnective tissue during this period,while during150days to180days were located in intralobularconnective tissue spreading around tubular of mammary gland and bigger than that in intralobularconnective tissue and occasionally were stained in purple. Wright-Giemsa staining showed thateosinophils scattered in the breast connective tissue with eosinophilic granular in cytoplasm dyed red.Thereis the lest number of eosinophils at birth day,while the lagest number at30days after birth,then decreasegradually. From150to180days after birth day,it is stable.