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QTL Mapping And Comprehensive Evaluation For Drought Resistance In Wheat Seedlings Stage

Posted on:2013-05-10Degree:MasterType:Thesis
Country:ChinaCandidate:K ChengFull Text:PDF
GTID:2233330374993696Subject:Genetics
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Wheat is one of the major food crops in China. Drought is one of the most importantbiotic stress factors that limit the yield and quality of wheat. Researches on geneticmechanism of drought resistance are important to the food security and sustainabledevelopment of China.A total of97QTLs were detected on16chromosomes,1A,1B,1D,2A,2B,2D,3B,3D,4A,4B,5A,5B,6A,6B,7A and7B. Of which,50QTLs on PEG,42QTLson CK and5QTLs On two conditions were determined, explaining5.63-37.30%of thephenotypic variations. The additive effects for54QTLs were positive with Shannong0431increasing the effects, while48QTLs were negative with Lumai21increasing the effects.Using a set of recombinant inbred lines (RILs) derived from the cross “Shannong0431×Lumai21”,14morphological and physiological traits related with droughtresistance were analyzed at the seedling stage under hydroponic cultivation with theosmotic stress treatment induced by PEG-6000. The drought resistance indices werecomprehensively screened by the principal components analysis, and the droughtresistance of the materials was comprehensively evaluated by the membership functionvalue analysis.The comprehensive screening results showed that five indices could be used as theindicators of seedling drought-resistant identification with the accumulative contributionrate of82.14%. The comprehensive evaluation results indicated that the drought resistanceof the male parent Lumai21was higher than that of the female parent Shannong0431.There was a great variation separation of the drought resistance among the population. Thedrought resistance of11lines was higher than the parent Lumai21,90lines was lowerthan the parent Shannong0431, and57lines was between the two parents.
Keywords/Search Tags:Wheat, Recombinant inbred lines (RILs), Drought resistance, Seedlingstage, Comprehensive evaluation, QTL location
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