Transformation Of Polygalacturonase Inhibiting Protein(PGIP)Gene From Capsicum Annuum Into Wheat And Maize And Resistance Evaluation Of The Transgenic Plants

Polygalacturonase inhibiting protein(PGIP) is a kind of specific proteins which can non-competitively inhabite endo-polygalacturonase(endo-PG) of pathogens and enhance resistance of plant. Many plants contained PGIP genes, but due to lower evolutionary pressure and fast speed of evolution of PGIP genes, some cereal crops lost these genes, but some plants still maintained these genes like solanaceae crops. In this study, we have transffered the PGIP gene into wheat and maize’s genome. In order that we can obtain transgenic wheat and maize which can show resisitance to fungal pathogens such as Gibberalla zeae, Pellicidaria sasakii. Exserohilum trucicum, Bipolaris maydis.The main results and conclutions are as follows,1. Construction of the plant expression vector pCBUPGIPThe PCR products of PGIP gene and the the binary vector pCAMBIA-Bar-Ubi were digested by same double enzymes, and ligated by T4ligase to generate the new construct pCBUPGIP.. The DNA of the recombinant new binary vector were purified, and some were used for biolistic transformation to generate transgenic wheat; and some were transferred into Agrobacterium tumefaciens strain LBA4404for maize transformation.2. Cultivation of the transgenic lines of wheat and maizeThe efficient regenezation systems were established after optimizing regenezation condition, and we succefully foster transgenic plants of wheat and maize.3. Resistant identification of fungal diseaseYangmai158transgenic wheat plants showed resistant to Gibberalla zeae and A188maize transgenic plants showed resistant to fungal pathogens of Bipolaris maydis, Exerohilum truicicum, and Pellicularia sasakii after inoculation with these fungi.