Proteomics Analysis Of Cerasus Sachalinensis Kom. Under Waterlogging Stress With Exogenous NO₃^-

On the basis of present study, this study was on going from2009-2011in root laboratoryof Shandong Agricultural University, Cerasus sachalinensis Kom. was used as experimentmaterials. The effects of exogenous NO₃^-on physiological indices and proteome in the rootsof cherry（Cerasus sachalinensis Kom） were studied. So result could offer enzyme activityand functionalprotein changes. The main results were as follows:1. NO₃^-treatment enheanced the content of autioxidant enzyme activities. Exogenous NO₃^-can significantly improve catalase（CAT）, superoxide dismutase（SOD），peroxidase（POD），ascorbate peroxidase（APX） activity, reduce the content of hydrogenperoxide.2. Under waterlogging, the content of malonaldehyde and proline were enhanced in the root,NO₃^-treatment enheanced the content of proline and reduced the content ofmalonaldehyde.3. The glutamate synthetase and nitrate reductase activity in roots of Cerasus sachalinensisKom. increased to the highest level and then declined gradually in both treatment andcontrol. Meanwhile, the glutamine synthelase activitiy decreased gradually, but threeenzymes activities of treatment were higher than that of control after using exogenousNO₃^-.4. Under waterlogging condition, the exogenous NO₃^-treatment decreased the content ofpolyphenol oxidase（PPO）, which were lower than control, while to the maximum, whichwere6.92%,21.77%,34.90%lower than that of control respectively when reached to themaximum.5. With two-dimensional electrophoresis, treatment and control all can be detected more than200kinds of root protein, of which12proteins were significantly different. Comparedwith control, two kinds of proteins quantity were significantly decreased,10proteinsexpression were significantly increased. All of the12proteins, most proteins wereincreased and molecular weight, isoelectric point were in the extensive region, but theircontent were very low.6. With the support of mass-spectrometric technique,we identified11protein:sheat shockprotein70, isoflavone reductase relatedprotein, triosephosphate isomerase, proteasome subunit alpha type-6, GS, Vf14-3-3cprotein, Small GTP binding protein, NDPK2, PGP1,polyphenol oxidase, allergen Pru av1and an unknown proteins. They played parts inmetabolism, signal transduction, hormonal regulation, etc.