| Sweet cherry (Prunus pseudocerasus Lindl.) is the important species for cultivation, which can bring much benefit to the economy. Since it is shallow roots, it is sensitive to waterlogging stress. And the sweet cherry plant is becoming weakening because of waterlogging, and even died. This constraint the cherry planting and brings production loss of the cherry industry. This article was studied on the basis of pro-research in root system lab, the Shandong key lab of fruit biology, Shandong Agricultural University, between 2006 ~ 2009 years. The effects of exogenous NO3- on photosynthesis, nitrogen and main carbohydrate, sucrose related enzymes, anaerobic respiration enzymes, alcohol and lactic acid contents in the roots of sweet cherry ( Prunus. avium.'Daihong') with P. pseudocerasus var Daqingye as rootstock under water-logging condition, were studied. So result could offer theory for sweet cherry to adapt to waterlogging on exogenous NO3- effect of physiological function. The main results are as follows:1. Under waterlogging condition, the leaf photosynthetic rates of sweet cherry decreased much more than that with exogenous NO3- treatment. On the third day, the photosynthetic rates of treated plant was the twice of control. Waterlogged for the fifth day, photosynthetic rate of control plant was 0.1μmol·m-2·s-1, and photosynthetic rate of treated plant was 2.5μmol·m-2·s-1.2. The results showed that under waterlogging condition, the contents of sucrose, glucose, fructose and starch in roots of sweet cherry increased significantly in the early period and then decreased in both treatment and control. Exogenous NO3- enhanced the contents of sucrose, glucose, fructose and starch in treated plant roots, which were higher than that of control. While to the maximum, contents of sucrose, glucose, fructose and starch in treated plant roots were 18.02%, 21.97%, 25.27% and 11.41% higher than that of control.3. The sucrose synthase disassemble activity in roots of sweet cherry increased to the highest level and then declined gradually in both treatment and control. Meanwhile, activities of the sucrose synthase synthesis, NI and AI decreased gradually, but four enzyme activities of treatment were higher than those of control after using exogenous NO3-.4. Exogenous NO3- treatment enhanced the contents of NO3- and NH4+ in roots of sweet cherry significantly in the early period of treatment and then decreased. When NO3- and NH4+contents reached to maximum, NO3- and NH4+contents of treated plant were 36.27% and 29.13% higher than those of control.5. Activities of nitrogen related enzymes such as NR, GS, GOGAT and GDH under addition NO3- were higher than those of control in roots of sweet cherry under waterlogging. And the activities of NR, GOGAT and GDH of treated planted were 34.58%, 16.30% and 38.87% higher than those of control.6. Under waterlogging condition, LDH, PDC and ADH activities of roots were enhanced, and contents of pyruvic acid, alcohol and lactic acid increased. Results showed that exogenous NO3- treatment enchanced activities of PDC and ADH, and inhibited activity of LDH. When activity of LDH reached the maximum, activities of control was 31.15% higher than that of treatment, at the same time, exogenous NO3- increased pyruvic acid and alcohol contents, decreased lactic acid content, and the lactic acid content of control plants was 27.83% higher than that of treated plants.7. Under waterlogging condition, additive exogenous NO3- enhanced marked activities of AlaAT, increased contents of Glu and Ala, and contents of Glu and Ala were 14.63% and 24.35% higher than those of control plants on the third day. |
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