| In recent years,with the development of animal feed industry,aquaculture expansion of the scale fluorine poisoning, the geochemical nature of the disease has been raised from fluorosis endemic fluorosis area began to spread to non-extension of fluorosis.The effect of feed fluorosis on livestock and poultry feed industry and human health impact can not be ignored. In previous research development of fluorosis both at home and abroad were centralized on skeleton organizations damage.Today the non-skeleton organizations damage has become a hot issue research. In order to further explore the sensitivity of non-Skeleton organizations and the damage mechanism of fluoride on animals the study add different doses of fluoride to the dog diet and detect the dynamic change of blood fluorine, enzyme activity, serum biochemical indicators and the impact of calcium ions.1Effect of different doses of fluorine intake on blood fluoride and serum xanthine oxidase activityWith the diets containing different levels of fluorine content of0mg/kg (control group),335mg/kg (low-fluoride group),760mg/kg (high-fluoride group),canines were fed continuously, results showed that:15days, serum fluoride concentrations in low-fluoride group and high-fluoride group were slightly higher compared with the control group.60days, compared with the control group, low-fluoride group serum fluoride concentrations increased by about55%, in high-fluoride group serum fluoride concentrations increased nearly doubled (P <0.01). The xanthine oxidase activity has also increased as the blood concentration of fluoride occur regularly changes. The first15days in the experimental, XOD activity in low-fluoride group and high-fluoride group increased21%,18%respectively (P<0.05).30days, XOD activity in the low-fluoride group and high-fluoride group both increased significantly. Compared with the control group the low-fluoride group increased63%, significantly faster than that with high-fluoride group (P<0.01). XOD activity in high-fluoride group increased20%compared with the control group (P<0.01). Changes of blood fluoride is not only time-dependent in canines feeding sodium fluoride, but also the level of fluoride concentration positively correlated. Serum xanthine oxidase activity is more sensitive to changes of blood fluoride. In the pre-trial serum xanthine oxidase activity increased with the blood fluoride rised. And then serum xanthine oxidase activity reduced after a first process.2The dynamic changes of serum indicators by different doses of fluorieWith the diets containing different levels of fluorine content of0mg/kg (control group),335mg/kg (low-fluoride group),760mg/kg (high-fluoride group),canines were fed continuously for the60days. During the trial the serum biochemical indicators were deteremined respectively on30days and60days. The results show that:30days compared with control group, high-dose group in ALT, AST, ALP, LDH significantly increased. ALB, A/G in the fluoride treated group significantly declined with the control group (P<0.01). TP in each group no significant difference between (P>0.05). GLO in the fluoride treated group significantly higher than the control group(P<0.01).60days, compared with the previous30days, ALT, AST, ALP, LDH in the fluoride treated group all experiencing decline. TP in low-fluoride group lower than11%of the control group. ALB in fluoride group were significantly lower than the control group (P<0.05). GLO, A/G in fluoride treated group are both declined.Compared with the control group, high-fluoride group was significantly declined (P<0.01).(Conclusion) Long-term intake of excessive fluoride can lead to lower enzyme activity,impaired immune function in dogs..3The dynamic changes of serum Calcium and Uric Acid by different doses of fluorieTo further explore the Interaction of Fluoride with Calcium and Inhibitory mechanisms of enzymes we study the dynamic changes of serum Calcium and Uric Acid By Fluorosis In Canine.36local dogs fed different levels of fluorine-containing feed were randomly divided into3groups,control (Omg/kg),low-fluoride group (335mg/kg), high-fluoride group(760mg/kg),12in each group for the60days. During the trial the serum calcium and uric Acid were deteremined respectively on15days,30days and60days.15days, compared with the control group, high-fluoride group serum calcium was significantly increased10.15%(P<0.05); serum uric acid in low-fluoride treated group significantly increased31.8%and increased27.59%in high-fluoride group with the control group (P<0.01).30days, compared with the control group, the low-fluoride group and high-fluoride group serum calcium increased17.53%,7.46%respectively (P <0.01). Uric acid in low-fluoride group increased54.55%and decreased36.07%in high-fluoride group with the control group (P<0.01).60days, compared with the control group serum calcium and uric acid all experiencing decline in high-fluoride group(P<0.01).In low-fluoride group serum calcium was declined16.41%(P <0.01) and uric acid no significant difference with the control group (P>0.05).Short-term intake of excessive fluoride can enhance serum calcium and uric acid. Long-term intake of excessive fluoride will lead to lower serum calcium and uric acid in canine. |
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