Study On The Effect Of Seminal Plasma On Cryopreservation Of Boar Semen

Boar sperm that was especially sensitive to temperature changes compared to other mammalian sperm, was extremely susceptible to temperature and damaged during the freezing process. For those reasons, the production of frozen semen was tough and greatly restricted the commercial application in swine. However, semen cryopreservation broke the time-space boundary of the semen used to promote joint breeding in multiple areas and shorten breeding intervals between generations. In addition, it improved the utilization ratio of breeding stock and saved a great quantity of feeding and management costs. The technology of semen cryopreservation is profound significance for development for modern animal husbandry. Therefore, clearly understand of the mechanism and developing key technologies of cryopreservation of porcine sperm, had become urgent problems in the production of pig all around the world.In order to evaluate the effect of the cryoprotectant, which contained different seminal plasma concentration, to activities ability and insemination ability after frozen and recovery of the boar semen, the semen was treated with10%,20%,30%cryoprotectant, respectively, in this experiment. The before and after frozen sperm vitality, sperm membrane integrity, egg crack rate and blastocyst after frozen IVF rate were monitored. While the control was TCG (Tris-citric acid-glucose) semen frozen foundation liquid with yolk. Results show that:the diluents added different concentration of seminal plasma, sperm motility rate before frozen had no differences in experimental groups(P>0.05), and the group added10%seminal plasma was higher in sperm motility rate (42.24±1.06%) than the other groups, significantly (P<0.05). Whatever before or after frozen, sperm plasma membrane integrity of the group with10%seminal plasma exceeded control group significantly (P<0.05). The experiment in vitro fertilization the group added10%seminal plasma as cryoprotectant increased the blastocysts rate (15.69±0.74%) compared to control group (12.54±1.04%), significantly (P<0.05), but cleavage rate had no difference clearly (P>0.05), and both cleavage rate and blastocysts rate were reduced significantly compared to fresh sperm (P<0.05). When frozen semen with10%seminal plasma thawed applied for artificial fertilization, pregnancy rate of the matching sow, number born and number born alive reduced significantly compared with fresh sperm (P<0.05).The results indicate that cryoprotectant with10%seminal plasma improved the ability of sperm resistance to freeze, but there are certain gaps in fertilization capacity between freeze-thawed sperm and fresh sperm. Therefore, the protection of cryopreservation by seminal plasma on boar semen required further study.