The Effects Of Selenium Deficiency On The DNA Methylation In The Tissue Of Chickens

DNA methylation is an important epigenetic mechanism of transcriptional control.DNA methylation plays an essential role in anumber of biological processes, such as gene expression,embryo development, cell differentiation, genomic imprinting, X chromosome inactivation and cell memory. Aberrant DNA methylation may lead to genetic instability and disease.selenium is a Essential trace element in human and animal, the level of its content related abnormal changes in DNA methylation. Experiments show that the lack of dietary selenium could lead to hypomethylation in liver and colon tissue and Caco-2cell lines in mice. It has not been reported the effects of dietary selenium on the level of DNA methylation in poultry organization.In the study, selenium deficiency model of chicken was copye by fed a Se-deficient basal diet. It has been examined the DNA methylation level by HPLC. It has been examined DNMTs and MBD2mRNA levels by the applications of RT-PCR. The main results were showed as follows:1.Developed and validated a specific High Performance liquid chromatography(HPLC) method for determination of DNA methylation in chicken tissue. HPLC condition:the chromatographic column was LC-10A vp plus (4.6mm×250mm, grain size5μm); the wavelength280nm; the mobile phase0.05mol/L KH2PO4(pH3.5); flow ratel.OmL/min; the column temperature30℃.2.Global DNA methylation level were lower in muscle tissues, brain tissues, immune tissues and livers of chicken in Se dificiency groups than that of control group.3.DNMT1、DNMT3A、DNMT3B mRNA expression level were lower in muscle tissues, brain tissues, immune tissues and livers of chicken in Se dificiency groups than that of control group.4.Demethylase MBD2mRNA expression level were increased in muscle tissues, brain tissues, immune tissues and livers of chicken in Se dificiency groups than that of control group.