Physiological Race Differentiation And Resistant Materials Screening And Genetic Transformation On Magnaporthe Grisea In Heilongjiang Province

Rice is one of the world’s major food crop, which originated in China. At present it is planted in114countries.China is the mainly rice produce country, its planting area is about1/4of total arable land area, the annual rice output is about45%of crop produces. Heilongjiang province is chinese important base for rice cultivation, rice safe production of Heilongjiang province is in relation to China political stability.Rice blast is a fungal disease,, and it is widely distributed in the world, especially in Asia and Africa. Currently85countries have reported the occurrence of rice blast. In China, Rice Blast is distributed in the place where rice is planted., Rice blast disease is also an important disease in heilongjiang province. It mainly affects the yield of rice.10%～30%of rice yield were lost in an epidemic year of rice blast disease,even40%～50%.and it influences rice quality. The epidemic of rice blast severely hazarded rice high yield and stable produce in heilongjiang province.Breeding and planting resistant cultivars is the most economic and effective measures for rice blast control, rice blast fungus variation is the main cause of the resistance loss of rice varieties, a comprehensive information of a dynamic composition of physiological race on rice blast fungus and screening of resistant materials is a basis for disease control.The physiological race monitoring of Magnaporthe grisea and screening of resistant materials play an important role for reasonable layout of resistant varieties.68isolates of rice blast fungus, Magnaporthe grisea, were collected in Heilongjiang province in2010and2011. We identificated the68isolates by using the differential hosts of China, At the same time, in this study, GFP was transformated successfully into the rice blast fungus, which provides an advantageous tool for the studies of the pathogenic mechanism of rice blast fungus and the mechanism of resistance of rice varieties.The results were as followed:1. This study showed that there were4groups17races by testing68isolates collected from the counties of Heilongjiang province in2010and2011by using the7differential hosts of China. Among them, ZA was the dominant groups and ZC1was the dominant race.2.30rice varieties were inoculated by rice blast fungus, the results show that,1Oof them were resistant varieties.9of them were middle resistant varieties, and11were susceptible varieties 3. The optimal the optimal pre induction time for Agrobacterium-mediated rice blast fungus trnaformation was8h, the total culture time wasl5h, co-cultured with AS concentration was200mol/L, culture temperature was25℃, co-cultured with pH value was5.6-5.8.4. GFP was transformated successfully into rice blast fungus using Agrobacterium mediated method in this study, verified the transformation. and then optimized genetic transformation method, make the transformation more time-saving.