The Proliferation And Colonization Of Sargassum Naozhouense Based On The Artificial Breeding

Sargassum is a multi-year renewable seaweed. It plays an important ecological role incoastal areas. Sargassum naozhouense has a lot of untapped potential in medicine, health,aquaculture and food industry. In the present study, the artificial breeding of differentsubstrate was carried out due to severe recession of Sargassum resources from May toOctober in2011. The artificial algal reef growth transplant and mariculture wereundertaken based on artificial breeding. This study was aimed at restoreing naturalSargassum resources, furnishing technical support for building large-scale seaweed,providing basic information for the marine ecological restoration, and supplying practicalmethods for germplasm conservation of large-scale economic seaweed. The experimentalresults are as follows:1. The artificial breeding of Sargassum naozhouense indoor were crossed the summerin mid-May to early October. Sargassum naozhouense was regular growth in hightemperature season cultivation indoor. There were25000young sporophyte seedlingsurvived attached at different substrate, crossings five months training. The average lengthwas27.3mm and the average adhesion density was27.9per cm~2.2. The growth stages, growth rate and adhesion density of Sargassum naozhouensewas observed and recorded in mid-May to early October crossing the summer period.Temperature has a major impact on Sargassum naozhouense seedling growth. The growthof seedlings which grown for14mm for30days below27℃is significantly more thanthe growth which increased only10mm for100days above27℃.3.Sargassum naozhouense seedlings adhesion density and growth rate were studied onfour substrate: nylon polyester blended curtains, coir rope, concrete pillars and yellowmud-brick. Concrete pillars was suitable for the sporophytes growth which reached to31.3mm; curtain was most conducive to the young sporophytes attachment; the yellow mudbrick is worst conducive to the young sporophytes attachment. The average number ofadhesion algae was22.8per cm~2at the end of breeding.4. The main predators and rivals in artificial breeding of Sargassum naozhouensewas investigated. The result show that Ulva lactuca and Caprella equilibra were the mainharm in artificial breeding; and epiphytic algae such as Diatom, Cladophora sp.,Enteromorpha prolifera and so on had a greater impact on development of Sargassumnaozhouense; the ingestion of predators such as Siganus Concatenalu, Ampithoe valida andCaprella equilibra was the main harm in artificial breeding at sea. 5. The effect on predators and rivals by different chemical or physical process wasstudied. The biological predators and epiphytic algae were inhibited effectively. Theconcentration of5%of ammonium sulfate solution with treating time5min restrained thegrowth of Cladophora sp.; the concentration of2%of citric acid with treating time5min，0.3%of acetic acid with3min and3%of ammonium nitrate with5min caused death ofepiphytic green algae such as Ulva lactuca and Enteromorpha prolifera，dipterex(640mg/L) with treating time10min had better effect on Ampithoe valida.6. The substrate at which Sargassum naozhouense seedlings attached was fixed byaccelerated cement on rocks. The growth of seedlings was observed and recordedanniversary. The proliferation of Sargassum naozhouense was succeeded, based on theartificial breeding indoor. There were42Sargassum naozhouense survived, and thesurvival rate was29.2%. The longest length of seedling was80.6cm. The proliferation ofSargassum naozhouense was proliferated by rhizoid after summer.7. The net bags with mature Sargassum naozhouense were fixed on the caisson whichhave no Sargassum naozhouense distribution in Nanshan, Xuwen. The number and lengthof Sargassum naozhouense was observed and measured. The colonization of Sargassumnaozhouense was succeeded, through reproducing by spores from wild Sargassum.Therewas11seedlings survived.8. The growth and survival rate of Sargassum naozhouense fixed by acceleratedcement, rope and epoxy glue and crysrocktiles mixed Tripoli was observed and recorded.There was51Sargassum naozhouense fixed survival, and the survival rate was18.9%,after80days. The length of seedling in rope was50.9cm and survival rate was30%.9. The effect of different water layer to the growth rate and adhesion density wasstudied. The result show that surface which had highest growth rate and adhesion densitywas most suitable for the growth of Sargassum naozhouense. The length was45.7mm andthe density was43.2%after15days. But there was a large number of Ulva and other greenalgae attached. The growth rate and adhesion density below surface60cm was lowest. Thelength was20.7mm and the density was15.3%. The length increased only1.2mm.10. The effect of different cultivated period in sea to the growth rate and adhesiondensity was studied. The growth rate and adhesion density cultivated in September wassignificantly higher than the August both indoor and in sea. It was beneficial to the growthof Sargassum naozhouense after high season. The length of Sargassum naozhouenseattached to the rope was only10.8mm in August, while it reached24.3mm in September.