Study On Physiological And Biochemical Of Turbot At Different Temperature, Salinity, And Their Interaction

The subject in the context of this study, take turbot juveniles as the researchobject. Using biochemistry, molecular biology, protemics, carry out a series of studiesto heat and salinity stress on the liver、nieren、skin、muscle and blood of turbot.Analyse the changes of antioxidant enzyme、Isoenzyme pedigree and the express ofimmunological factors, as a best suited to the environments in which the fish are to befarmed.Two-factor-intersecting experiment was designed to evaluate the effects oftemperature(17℃、20℃、23℃、25℃、28℃) and salinity(5‰、10‰、20‰、30‰、40‰) on the activities、death rate and antioxidant enzymatic activities in the mucus、blood、gill and liver of juvenil Scophthalmus maximus. The results showed that thefish had a good ingestion in the group of17℃、30‰and17℃、20‰. Bothtemperature and salinity had significant effect (P<0.05)on the activity of SOD、CAT、GP-X. The activity of SOD、CAT and GP-X changed like broken line with theincrease of temperature and relatively higher at20℃and23℃. The activity ofmucus、blood、gill and liver SOD、CAT、GP-X increased as the salinity increased ordecreased from30‰,and had a lower activity at30‰while higher at5‰and40‰.The temperature and salinity interaction had significant effect (P<0.05) on theactivity of SOD、CAT and GP-X.Polyarylamide gel electrophoresis test was used to study the expression of SOD、EST、MDH、GDH isozyme in liver、skin、muscle and gill of turbot at13℃、20℃、23℃、25℃、28℃and5‰、10‰、20‰、30‰、40‰. The results were as follows:the expression of heat and salinity have effects on all isozyme which change thebrightness of isoenzyme pedigree, the activity of SOD、EST、MDH isozyme boost upwith the temperature then begin lower at28℃. The temperature has different effect onGDH in different tissue. The activity of SOD、EST、MDH isozyme of four tissue andGDH of the gill boost up compared with control group when the salt changed, whiletrail off in liver、skip and muscle. To use RT-PCR method determined the immune-related factors includingimmunoglobulin M (IgM), hepcidins, lysozyme heat shock protein70, heat shockprotein90,C-t type lectin, Lily-type lectin in liver、nieren and skin under the conditionof20℃、23℃、25℃、28℃and5‰、10‰、20‰、30‰、40‰. The results showed:The temperature has dramatically effect on all the immune-related factors of threetissue, when the salinity changes it has low effect on immune-related factors of theliver, while makes much difference in skin, the immune-related factors of the nierenhave higher expressions at40‰.