The Study And Application Of Bioflocs As Aristichthys Nobilis Richardson Bait

The bio-floc technology (Biofloc technology, BFT) is a new culture technology byinput lots of feed into aquaculture water in order to supply organic carbon, maintaindefined carbon and nitrogen proportion regulating microbial community to convertammonia nitrogen into bacterial protein and improve feed utilization, driveling fromactivated suspension technique. BFT is namely heterotrophic bacterium, utilizingmicroorganism to combinative redundant nutrients existed water in order to formnutrients source feeded cicely aqua animals, and able to substitutive feed or reducingfeed level, and solve the problem of detritus and feed detention, so that control waterquality and improve protein utilization, which play a important role in aquaculture.BFT have applied to aquaculture, and have more researches, especially shrimp、tilapiaand other omnivorous economic aquatic animals. Aristichthys nobilis Richardson isfilter-feeding fish, only as mixed cultured species, In traditionally, we via fertilized(N、Pand organic matter) to culture plankton feeded for Aristichthys nobilis Richardson. Butin this way bait volume limit Aristichthys nobilis Richardson growth and yield.Therefore, to explore a new culture bait way which improve produce is very important.In order to BFT efficiency for “herring, grass carp, sliver carp and bighead carp”, in thisstudy, by Aristichthys nobilis Richardson as object, with glucose as carbon source, thenaccording to fixed proportion to add carbon source culturing biofloc, to evaluate theeffects of the bioflocs technology (BFT) on growth performance, muscle composition,muscle amino acids composition and content, non-specific immunity indices and waterquality of the bighead carp (Aristichthys nobilis), and to further understand biofloc asbait effect, provide a new ideal for culture model. Result is as follows:1. Stocking Aristichthys nobilis to biofloc culture group(TSS level maintain100mg/l)、fertilize culture group[add urea7g(N46%) and superphosphate3g(P2O516%),maintain zooplankton level8-10ind/l] and cage hanging water group[cage hang grasscarp pond upper water(1m), maintain zooplankton level3-5ind/l]. After56days, Biofloc culture group treatments terminal total weight and individual average weightwere significantly higher than that in the fertilize culture group and cage hanging watergroup (P＜0.05). Average daily gain (ADG) and specific growth rate (SGR) in bioflocgroup were significantly higher than that in the fertilize culture group and cage hangingwater group (P＜0.05), No significant difference in the final body length were observedamong all groups (P＞0.05).2. Stocking Aristichthys nobilis to biofloc culture group(TSS level maintain100mg/l)、fertilize culture group[add urea7g(N46%) and superphosphate3g(P2O516%),maintain zooplankton level8-10ind/l] and cage hanging water group[cage hang grasscarp pond upper water(1m), maintain zooplankton level3-5ind/l]. After56days, Crudeprotein content of muscle in the biofloc culture group was significantly higher than thatin the fertilizer culture group and cage hanging water group(P＜0.05), but in the crudelipid of muscle in the biofloc culture group was significantly lower than that in othergroups (P＞0.05). No significant differences in the moisture and ash of muscle wereobserved among all groups (P＞0.05).3. Stocking Aristichthys nobilis to biofloc culture group(TSS level maintain100mg/l)、fertilize culture group[add urea7g(N46%) and superphosphate3g(P2O516%),maintain zooplankton level8-10ind/l] and cage hanging water group[cage hang grasscarp pond upper water(1m), maintain zooplankton level3-5ind/l]. After56days, Serumlysozyme (LSZ), nitric oxide synthase (NOS) and acid phosphatase (ACP) activity inbiofloc culture group were significantly higherthan that in the fertilizer culture groupand cage hanging water group. No significant difference in the activity of alkalinephosphatase (AKP) was were observed among all groups (P＞0.05).4. Stocking Aristichthys nobilis to biofloc culture group(TSS level maintain100mg/l)、fertilize culture group[add urea7g(N46%) and superphosphate3g(P2O516%),maintain zooplankton level8-10ind/l] and cage hanging water group[cage hang grasscarp pond upper water(1m), maintain zooplankton level3-5ind/l]. After56days, theammonia nitrogen content in the biofloc culture group was slightly lower than that inother groups.5. Use biofloc(CP29.14%) and compound feed(CP32%) to feed Aristichthysnobilis, After56days, to compared with feed with common compound feed, theAristichthys nobilis which feed with biofloc, the final individual weight and weight gainrate improved8.67%(P <0.05) and141.49%(P <0.05), respectively. The terminal total weight and fatness in biofloc groups significantly (P <0.05) higher than control group,in body length and physical indicators (including the internal organs and liver), nosignificant differences in the biofloc groups (P>0.05).6. Use biofloc(CP29.14%) and compound feed(CP32%) to feed Aristichthysnobilis, After56days, the muscle of Aristichthys nobilis which feed with compoundfeed and biofloc, the composition of hydrolysis amino acid were no difference(P>0.05),both containing16amino acids, the biofloc group total amino acid (15.249±0.557)%,and compound feed group (15.175±0.780)%. The total4kinds of delicious aminoacids account for (6.215±0.136)%in group of biofloc, which improve1.57%comparedwith the compound feed group.