Study On The Correlation Between The Microflora Of Honeybee (Apis Mellifera) And Plant Diseases

In this research, the microecological of Apis mellifera ligustica was studied. Firstly, the floras of bacteria and fungi in Apis mellifera ligustica during the litchi florescence period (April-May2008), the summer period (July-August2008), and the winter period (January-February2009) were investigated. And then, the antagonistic bacteria from Apis mellifera ligustica were screened. The results were as follows:Obtained14,9,10bacteria isolates from the adult worker bees of Apis mellifera ligustica in litchi florescence, summer, and winter, respectively, those bacteria were the genera Bacillus, Proteus, Citrobacter, Pseudomonas, Serratia, Pantoea, Enterobacter and Klebsiella. The dominant microflora of different periods was distinct. The results showed that Bacillus and Proteus were the dominant microflora in litchi florescence, while Citrobacter, Proteus and Bacillus were the dominant microflora in summer, Citrobacter, Proteus and Bacillus were the dominant microflora in winter. The sequences of these33strains were submitted to GenBank, and the acession number was GQ487534-GQ487566. These sequences have been published in the database.The281isolates belong to11genera of fungi were isolated from the body surface of Apis mellifera ligustica adult worker bees. Those strains included Penicillium, Paecilomyces, Hyalodendron, Cladosporium, Aspergillus, Trichoderma, Alternaria, Mucor, Rhizopus, Fulvia, Humicola. The genera of Penicillium and Hyalodendron were the dominant populations in the three periods. In addition,107isolates belong to6genera of fungi were isolated from Apis mellifera ligustica intestinal. Those strains of fungi include Penicillium, Hyalodendron, Pleiochaeta, Cladosporium, Bipolaris and Curvularia. The genera of Pleiochaeta.Cladosporium and Penicillium were the dominant populations.Two strains of Bacillus subtilis, HB0501and HB0709, which displayed strong antagonism to plant pathogens, were isolated from the intestinal of Apis mellifera ligustica. The results showed that HB0501had a stronger antagonistic effect than Colletotrichum nicotianae. Virulence tests indicated that Penicillium and Alternaria, which had been isolated from the surface of Apis mellifera ligustica, were nonpathogenic fungi.