| An experiment was conducted to study on the the flavonoids and fatty acids from two kinds of wild Changbai Mountain Ligularia, for L. jaluensis and Ligularia intermedia Nakai. According to the literature search, we determined the fatty acid analysis of the flavonoids contained in both leaves and flowers. In order to process optimization for the two Ligularia flavonoids extraction rate,used the response surface method; Antioxidant and antibacterial activity of referred to the flavonoid compounds; The petals are extracted fatty acids were identified by GC-MS; the flavonoids were isolated by HSCCC method.1. The study of the extraction rate of the L. jaluensis and Ligularia intermedia Nakai. First single factor of the solid-liquid ratio, ultrasonic time, ethanol concentration, ultrasonic temperature experiment, the software and choose which three of the factors response surface optimization analysis. Final L. jaluensis Flavonoids reach was0.8004mg/g, when the liquid ratio32.95mL/g, the ethanol volume fraction of86.60%ultrasonic time of44.51min. Factors that affect the extraction rate of total flavonoids in order was; Liquid ratio is greater than the ultrasonic time and greater than the ethanol concentration. Final Ligularia intermedia Nakai Flavonoids reach was0.6540mg/g, when the liquid ratio34.28mL/g, the ethanol volume fraction of68.80%ultrasonic time of32.56min. Factors that affect the extraction rate of total flavonoids in order was: Liquid ratio is greater than the ultrasonic time and greater than the ethanol concentration.2. Antioxidant and antibacterial activity of referred to the flavonoid compounds. The experiment that anti-hydroxyl radical:Vc> Ligularia intermedia Nakai> L. jaluensis; In superoxide anion: L. jaluensis>Vc> Ligularia intermedia Nakai; In the anti-DPPH radical:in the case of low concentration of two Ligularia, they lower than Vc. in the case of higher concentrations, Vc> L. jaluensis> Ligularia intermedia Nakai. As antibacterial agents, the results can inhibit Staphylococcus aureus in Escherichia coli. Bacillus subtilis and gold, with a certain degree of antibacterial activity completely aerogenes did not inhibit the antimicrobial effect of the two less.3. The extracted fatty acids were identified by GC-MS method. L. jaluensis identified a total of19kinds of ingredients. L. intermedia Nakai identified a total of35kinds of ingredients.Between the two components in common, but there are many different.4. The flavonoids were isolated by HSCCC method. First to use UV analysis to get the best solvent system for HSCCC chloroform-methanol-water (4:3:3). On the machine to get a fraction, the fraction will enter the HPLC analysis found that the fraction is not a single compound, but also need the subsequent separation. |
