CPT Gene Expression And Analysis In Muscle Tissue Of Goat And Sheep

In this study, the goats and sheep for the materials extracted from the heart, liver, spleen, and skeletal muscle total RNA, cloning, and sequencing of the sheep and goats, part of the cDNA sequence of CPT1A、CPT1B and CPT2gene, real-time quantitative PCR in goats and sheep tissue expression.The QPCR technical CPT1A. CPT1B and CPT2gene partial cDNA sequences, the nucleotide sequence by cloning, sequencing, QPCR, respectively.304bp.232bp and214bp. Bioinformatic analysis of CPT1A, CPTIB and the CPT2nucleotide sequence found between goats and sheep CPT1A, CPTIB and CPT2gene sequence of bovine conservative. Goats and sheep CPT1A gene and the similarity of the cattle up to97%,99%of the similarity of the goat CPT1B gene of sheep and cattle are the CPT2gene with similarity of cattle up to98%.Using Quantification PCR,(QPCR) analysis of carnitine palmitoyl transferase enzyme CPT gene expression in different tissues of goats and sheep. Sheep of CPT (1A,1B,2) gene tissue expression spectrum results show that these three genes were expressed in the detection of43organizations, which shows that the CPT (1A,1B,2) gene on the sheep’s body is a widely expressed genes. CPT1A.1B,2) gene expression in goats and sheep tissue differences may be associated with each gene expression patterns, different types of muscle tissue fibers, different content of fat deposition and other factors’...