The Comprehensive Utilization Of The Cottonseed Cake Meal By Multi-strain Mixed Fermentation

Cottonseed cake form cottonseed is full of botanical protein resource,and itsprotein content has a range from33%to45%.Utilization value of cottonseed cakeprotein is seriously affected because of free gossypol toxicity.This study decreasingfree gossypol content and increasing true protein content on cottonseed meal by thetechnology of mixed microorganism by Aspergillus niger, Aspergillus oryzae andSaccharomyces cerevisiae.The plant small peptides which prepared by enzymatichydrolysis has been low temperature and low acid hydrolyzed to obtain thecompound amino acid liquid.Then after adding the hydrolysis residue to raw stuff,accessing several probiotics to it for preparing the bioactivity feed.In present study, we investigated the fermentation conditions of cottonseedcake detoxification by mixed solid state fermentation by Aspergillusniger,Aspergillus oryzae and Saccharomyces cerevisiae.The results show that,theoptimal conditions for mixed fermentation were as follow: inoculum10%,humidity85%, material dry base: water=1:1,fermentation at34℃for48h.The removal rateof free gossypol in cottonseed cake was96.2%and crude protein content wasincreased from42.8%to44.6%.Enzymatic hydrolysis condition of cottonseed cake which has been compoundmicrobial fermentation.Enzymes which produced by microbial metabolismfermentation hydrolyzed the cottonseed cake to obtain liquid small molecularpeptides or small molecular peptide powder.The results show that, the optimalconditions for enzyme hydrolysis were as follow:the mixture of formationcottonseed cake and2.5times of water has been put in the thermostatwaterbath at55℃for12h,pH was kept at6.5during the hydrolysis process.The final hydrolysisrate was11.24%, and the content of free gossypol in liquid small molecular peptidewas19.9mg/Kg (0.00199%).The small peptide those molecular weight is less than1000Da accounted for93.4%of the total.Moreover,the small peptide those molecular weight is more than2000Da only accounted for1.8%.We studied the technology of low temperature,low acid hydrolysis filterresidue which has been hydrolyzed by enzymes.The results show that, the optimalconditions for low temperature and low acid hydrolysis were as follow:thehydrolysis at90℃for12h,add acid content was7.5%.The amount of amino acid inhydrolyzate was1.52g/100ml.The free gossypol content was15.4mg/Kg(0.00154%),and rate of amino acid production was58.6%.At the same time3-chloro propanol was not been detected in hydrolyzate.Part of raw materials were added to the filter residue which has been acidhydrolysis,and accessed probiotics such as Bacillus, Bacillus subtilis,Yeast,Lacticacid bacteria,then produced probiotics bioactivity feed.We investigated thefermentation technology of multiple strain mixed formation and the results showedthat the optimal fermentation conditions were as follow: the amount of water in rawstuff was50%, accessed Bacillus (E01: E02=1:1) after fermentation for12h,at thattime the inoculation amount of Lactic acid bacteria was10%,fermentation at32℃for48h.The content of crude protein in feed after been fermented was increasedfrom27.58%to37.92%,and the content of amino acid nitrogen is1.88%.Thenumber of Yeast live bacteria counting up to2.2×108/g(dry basis),and live lacticacid bacteria was up to2.30×108/g(dry basis),and amount of bacillus was1.84×109/g(dry basis).At the same time the free gossypol content was45.2mg/Kg.