| Ascorbic acid is a kind of hexose lactone compounds which the plants and most animals can combine in their body. Ascorbic acid have strong antioxidant activity, is a common antioxidant, and all green plants can be synthesized ascorbic acid. The total flavonoids is a large class of compounds containing a C6-C3-C6structure of plant secondary metabolites, have significant antioxidant function of the second largest plant polyphenols. Ferulic acid is a phenolic acid, could effectively eliminate the peroxide, hydroxyl radicals, oxygen free radicals, peroxynitrite, has been shown effective antioxidant properties. Wheat as the most widely plant of food crops, the people only focus on the use of its seed all the time, while ignoring the natural resources of wheat seedlings. Wheat grass is rich in antioxidants such as ascorbic acid, vitamin E, flavonoids, phenolic acids and some antioxidant enzymes. The experiment determined the content of ascorbic acid, flavonoids and ferulic acid in wheat seedlings with the wheat growing stage changes, the antioxidant activity of three antioxidant substances and synergistic antioxidant activity also analysed. The main content is as follows:(1) The content of three antioxidant substance in wheat seedling were measured and analyzed with changes of wheat growth. Use spectrophotometry, spectrophotometric and high performance liquid chromatography to determine the content of ascorbic acid, flavonoids and ferulic acid in wheat seedling, respectively. Which ascorbic acid content showed a general trend of lifting movements,1-2peak in the growth period; The content of total flavonoids was the highest in the analysis of the three substances, and its overall increase of content was relatively stable. The ferulic acid content in the entire growth period also showed a growing trend, and its growth rate maximum growth after two weeks in the wheat seedling.(2) Compared the content variance of the three antioxidants in wheat seedling. In six wheat cultivar tested, ascorbic acid content of strains030482overall than the other five strains, LM2total flavonoid content in wheat leaves was highest in the wheat lines tested, up to9.41mg·g-1. the average concentration of8.37mg·g-1. The highest content of ferulic acid was LM07-19, up to0.52mg·g-1, the average content of0.39mg·g-1. Compared the content of three substances in wheat seedling between2010and2011, the three substances in the wheat seedling content of the overall trend was the same, but the content of the number were differences.which related with environment that wheat experienced.(3) Analyzed antioxidant capacity of wheat juice and wheat seedling extracts.Antio xidant activities of wheat leaves extracts and wheat juice were evaluated using2,2-diphenyl-β-picrylhydrazyl(DPPH) assay and2,2’-azino-bis-3-ethylbenzthiazoline-6-sulp honic acid (ABTs) assay. Wheat juice’s antioxidant activity was greater than the ant ioxidant activity of wheat seedling extracts, because the wheat seedling contain a lo t of effective antioxidants can not be fully extracted and solved in the extraction so lvent. Compared the antioxidant activity of wheat seedling extracts, the antioxidant activity of ethanol extract was greater than water extract, this was because most of the antioxidants can be dissolved in ethanol, there were fewer antioxidants in wate r.(4) Analyzed of the three antioxidants synergistic antioxidant capacity. DPPH· method for the determination of the antioxidant activity of the compound of antioxidants, using factorial analysis and regression analysis discussed the synergies of the composite antioxidant activity. The results showed that the order of antioxidant scavenging free radical capacity was flavonoids> ferulic acid> ascorbic acid. Collaborative analysis of the three substances on the total antioxidant capacity, which rutin made largest contribution to total antioxidant capacity, followed by ascorbic acid, ferulic acid was the minimum. There were very significant synergistic antioxidant effect among the three components. |
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