The Association Analysis To The Rabbit MyD88Polymorphism And Expression With The Susceptibility To Intestinal Inflammation

Currently the polymorphisms of TLR4gene have shown the association with destive disorder,While MyD88as a downstream transduction signaling molecules to recognize TLR4. have been never studied in the association between the polymorphism and enteritis. In our study, case-control group of284individuals (case group149and135healthy controls) used the HRM to genotype for determine the site effect in the rabbit enteritis. Subsequntely RT-PCR was used to detect the expression of MyD88in Sacculus rotundusto associate with the severity of the enteritis as well as the genotype. The results as following:1.By direct sequencing ten variation sites were screened, of which only one site in the coding region of exon4. c.816C> T mutation, silent mutations.Others located on introns., including mutation of g.670C>T in intron1,mutations ofg.1213G>C, g.1217G>C and g.1252C>Tin intron2, mutation ofg.1576C>Tin intron3andmutations ofg.1709A>G, g.1763C>T, g.1765T>G and g.1770C>Tin intron4.2.expanding the sample population. HRM technical analysis showed that the C.816C> T allele T was the dominant allele,which the frequency was0.72. meannwhile TT genotype frequency (0.57) was also higher than the other two genotypes CT (0.22) and CC (0.21). Fisher exact test. c.816C> T variation point allele and genotype frequencies difference between Case and Control group was significantly (p<0.05), the C allele of the c.816C> T mutation sites risk calculation (contorl VS case), OR=0.6673, p=0.0245.95%confidence interval0.4688-0.9499, suggesting that in the MyD88gene allele C has a protective effect for enteritis in rabbits.3.By homogenization treatment of Expression, found that the relative expression levels of the severe enteritis group (S,1±.1283) was significantly higher than the healthy group (H,0.5187±0.1473, p=0.0074) and a mild inflammatory group (M.0.5673±0.1283, P=0.0065).In severe intestinal inflammation, the relative expression levels of genotype TT (1±0.1070) was significantly higher than the genotypes of the CC (0.4440±0.1587. P=0.0088), higher than the TC genotype expression (0.7888±0.1341), indicating that the variable sites c.816C> T was susceptibile in enteritis.Resequencing of the coding region of MyD88variants c.816C> T, case-control study and expression detection showed that the MyD88gene as a candidate susceptible gene in rabbit enteritis, which is the successor to conduct depth study the biological significance and laying a solid foundation to disease resistance breeding.