The Analysis Of Allelic Variation At Low Molecular Weight Glutenin Subunit Glu-A3and Glu-B3Loci

In this study, the distributions of Glu-A3seven alleles about228Chinese micro corecollections are identified by PCR.225varieties contain the Glu-A3seven alleles,however3varieties are tested unknown allele type. About these species, the number ofspecies which containing a and d alleles is larger than other types. The percentage of aand d alleles are31.1%and25%respectively; The ratio of b,c,e,f,g and unknownalleles are14%,2.6%,11.4%,5.3%,9.2%and1.3%respectively. The distributions ofGlu-B3locus9alleles in Chinese micro core collections are determined by PCR. Weknow that nine known alleles appear in223varieties and j allele appear in5varieties.The number containing i, a, d three alleles of species is the most. The percentages are21.5%,15.8%and12.7%; And the ratio of f,g,b,e,c,j,h alleles are11.8%,11.8%,9.6%,7.5%,5.3%,2.2％,1.8%respectively.The distribution trends of7alleles at Glu-A3locus between improved cultivars andlandraces are basically consistent. The percentage of a and d allele are27.7%and24.1%respectively in improved cultivars and33.1%and25.5%respectively in landraces. Thenumbers are significantly higher than other subunits‘percentage；The distributions ofthese7alleles among three wheat growing zones are greatly difference. The d allelefrequency in the winter wheat growing zones (28.6%) is significantly higher than thespring wheat growing zones (3.7%), and the e allele frequency in the winter wheatgrowing zones (6.1%) is lower than the spring wheat region (25.9%).Glu-B3locusalleles‘distributions are not only different among three wheat growing zones, but alsodifferent between improved cultivars and landraces. The percentage of d allele inimproved cultivars(19.3%) is higher than the percentage in landraces (9.0%). Thepercentage of a allele in improved cultivars(6.0%) is lower than the percentage inlandraces (21.4%). The d allele frequency in the winter wheat growing zones (27.1%) issignificantly higher than the spring wheat growing zones (0.0%), and the g allelefrequency in the winter wheat growing zones (5.1%) is lower than the spring wheatregion (35.3%).Three GluB3-3type sequences were cloned. One sequence is most similar toGluB3-32sequence (GenBank: EU369716.1). The similarity is up to99%. Comparedwith GluB3-32sequence, it has a SNP. But this SNP doesn‘t lead to the changes of theircorresponding aminophenol sequence. One sequence is also most similar to GluB3-32sequence (GenBank: EU369716.1)(similarity99%). Compared with GluB3-32sequence, it has2SNPs. One SNP doesn‘t lead to the changes of their corresponding aminophenolbut the other one does. The aminophenol type is from P to Q. The change occurred in theN-terminal repeat region. The last sequence with the highest similarity99%comparedwith GluB3-33(GenBank: EU369717.1) has a SNP. It also lead to the amino acidsequence changes. The deduced amino acid sequences show that three kinds of subunitsare belong to LMW-m type。...