The Anticoccidial Efficacy And Pharmacokinetics Of Sulfachloropyrazine Sodium-Diaveridine Suspensions In Broilers

Sulfachlorpyrazine (SPZ) is one of SAs which have the strongest Anticoccidial effect,its peak period is the second generation schizont of coccidian, and it has certain effect to the first generation schizont too. As the Sulfa synergist, Diaveridine (DVD) Combined with Sulfachlorpyrazine can block the folic acid metabolism of insect body from two different links, and enhance their anticoccidial efficacy. By studying the pharmacokinetics characteristics of Sulfachloropyrazine sodium-Diaveridine Suspension in the chicken and its treatment effect of this product to the Artificial infection of chicks with Eimeria tenella. this test is used to guide its clinical administration as well as the research and invention of new drug agents and provide reference for the future residue eliminate research.1. Pharmacodynamics of Sulfachloropyrazine sodium and Diaveridine Suspension in broilersIn order to verifying the anticoccidial effect of Sulfachloropyrazine sodium-Diaveridine Suspension, one hundred and eighty healthy chickens of15days old were divided into12groups,and infected artificially primary Eimeria tenella.Apart from not infected with non-treatment group,the others were infected by the way of pouches with the dose of12×104.The trial period is nine days, under the prescribed dose of the test and control drug were administed continuously3or5days by drinking water.All the chickens were weight, dissected and checked in the8th day after infection,then observed the cecum lesions, and took the cecal contents to calculate the number of oocysts in the cecum. The test utilized the indicators including bloody stools score, survival rate, lesion score and lesion values, oocyst production and oocysts value, anticoccidial index and so on to judge the drug efficacy and used Duncan’s new multiple range method to conduct significant analysis of the average weight gain and the average lesion score of each experimental group.The results suggested the infected symptoms of chicks did not appear or all disappeared when Sulfachloropyrazine sodium-Diaveridine Suspension was administed continuously3to5days by drinking water under the dose of150to300mg/L, the ACI was greater than180which belonged to efficient level,and the treatment effect was significantly better than the Sulfachloropyrazine sodium Soluble powder.2. Pharmacokinetics of Sulfachloropyrazine sodium and Diaveridine Suspension in broilersTirty-six broilers of35days old were used in this study,and randomly divided into three groups.The dose of first group of broilers were15mg/kg b.w. through single intravenous administration of Sulfachloropyrazine sodium and Diaveridine solution, the second group were30mg/kg b.w. through single oral administration of Sulfachloropyrazine sodium and Diaveridine Suspension, the third group were30mg/kg b.w. through single oral administration of Sulfachloropyrazine sodium soluble powder. Blood samples were collected according to the scheduled time and the concentration of the drug in plasma were determined by high performance liquid chromatography (HPLC). Pharmacokinetic parameters were obtained by the calculating of The Kinetica pharmacokinetic analysis software on the basis of the measured blood drug concentration-time data.After single intravenous administration of Sulfachloropyrazine sodium and Diaveridine solution, the main pharmacokinetic parameters of SPZ were as follow:t1/2β12.04h, MRT18.49h, AUC229.52(mg/L)·h, CLB1.13ml/(min·kg),Vz1.17L/kg; The main pharmacokinetic parameters of DVD were as follows:tI/2β6.86h, MRT9.08h, AUC2.70(mg/L)·h, Vz35.63L/kg, CLB69.92ml/(min·kg).After single oral administration of Sulfachloropyrazine sodium soluble powder, the main pharmacokinetic parameters of SPZ were t1/2β10.38h, MRT19.68h, Tmax6.17h, Cmax19.03mg/L, AUC395.57(mg/L)·h, CLB1.47ml/(min·kg), Vz1.07L/kg, F86%.After single oral administration of Sulfachloropyrazine sodium-Diaveridine Suspension,the main pharmacokinetic parameters of SPZ were as follows:Thalf17.50h, MRT28.97h, Tmax10.50h, Cmax6.44mg/L, AUC178.26(mg/L)·h, CLB2.91ml/(min·kg),Vz4.68L/kg, F39%.The main pharmacokinetic parameters of DVD were as follows:Thalf17.51h, MRT27.89h, tmax6.08h, CmaxO.25mg/L, AUC2.18(mg/L)·h, Vz110.81L/kg, CLB137.67ml/(min-kg), F40%.The results showed that the absorption of Sulfachloropyrazine sodium-Diaveridine Suspension after oral administration were not rules, and the individual differences was significant. Comparing to Sulfachloropyrazine sodium soluble powder,the absorption and elimination of SPZ in Sulfachloropyrazine sodium-Diaveridine Suspension was slower, and the bioavailability was lower; the absorption of DVD after oral administration was little,and its distribution in body was wider than SPZ, but because of the high body CLB, the DVD was eliminated very quickly.