| Strawberry (Fragaria x ananassa Duck.) is a worldwide fruit. Due to the rich fruit nutrition, certain medical value and high economic value, it has been widely cultivated throughout the world. Strawberry fruit belongs to the berries. Its skin is too thin to suit the long distance transportation, and the postharvest ripening and senescence particular fastly, which results in its very short shelf life (usually less than a week). These problems seriously restrict the economic benefits, and become a big difficult in production practice.At present, research on epigenetics is mainly used in human disease and aging, less research in plant development and senescence is reported, especisaly in postharvest fruit senescence Study on the epigenetic regulation mechanism of miRNA in senescence of non climacteric fruit strawberry, possess great application value and economic value for new varieties of strawberry breeding. The present results are as follows:(1) Through microRNA sequencing analysis on strawberry fruit:there are11pre-miRNAs corresponding to16unique mature microRNA originating from other plant pre-miRNAs has been discovered. Among them,85p or3p new mature microRNAs has been first time detected in this experiments;60novel pre-microRNA of72microRNAs have been reported, among them we found18pairs of3p/5p microRNAs; there are1157pre-miRNAs corresponding to1224unique mature miRNAs has been first reported in our experiments. Among them we found177pairs of3p/5p microRNAs.(2) By using plant tissue culture technology to optimize in vitro regeneration breeding system of strawberry, we obtained optimum culture medium for different stages of strawberry culture, such as strawberry meristem culture, callus formation, inducing shoots. These results provided a sterile material for experimental transgenic strawberry.(3) We constructed the overexpression vector pSN1301-PC-5p-229923, and transformed into strawberry leaves via Agrobacterium tumefaciens, and obtained the transgenic lines. |
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