Research Of DNA Cytosine Methylation Pattens And Levels In Maize Hybirds And Their Parents

Maize is one of the major food crops in the world. Production of maize increases its outputwith the heterosis. Exploration and research should be continued in order to make better use ofheterosis. In the research of epigenetic, DNA cytosine methylation affect organisms’ phenotypicchanges and it is one of the ways of genomic DNA in eukaryotic cells modification processing.DNA methylation exists in many higher plants and animals as an important form of epigeneticmodification. And DNA methylation in the regulation of gene expression and maintenance ofgenomic stability play a important biological regulatory role. In this study, research of heterosisin maize during development is commencing in differences of DNA methylation differences, inorder to reveal the distribution regularity and genetic role of DNA methylation in maize genome,and study methylation of maize growth, and explore the possible relationship between DNAmethylation and heterosis. Maize heterosis provides a theoretical basis to cultivate strongadvantage of new maize varieties.In this study, the MSAP method was studied and analyzed the methylation status of thethree maize hybrids and their parental immature embryo, endosperm, leaves, roots. The result isthat immature embryo, endosperm, leaves, roots methylation are outside of cytosine methylation.And the maize hybrids’ level of unmethylated sites is higher than the mid-parent value. Thedegree of methylation may cause the decrease expression of certain genes, so as to manifest thedominant trait. Jida101methylation level is higher than other varieties, so that, a certain degreeof methylation differences may exist between the different varieties. DNA methylation level inmaize hybrids is lower than the phenomenon of the inbred lines, indicating that the dominanttraits associated with reduced DNA methylation in hybrids, there was no difference between thereciprocal cross materials. We verify the genetic variation patterns of DNA methylation betweencorn parents and hybrids with the way of Southern Blot.We recycled and sequenced222genetic variation bands include24functional sites. In thesesites,12from embryoes,5from endosperms,4from the root,3from the leaves. Homologoussequence analysis showed that the24fragments with known functional genes homologous.Including: maize transporter gene, anti-rust resistance genes, plant growth regulatory factor,reverse transposase gene, quality-male sterility recovery factor, serine-threonine kinase,transport inhibition response protein, heat shock protein, ATP synthase gene, and so on. Selected and cloned with ATP synthase synthesis and growth factor-related gene NM001154086andAY530951which about DNA methylation. Then we studies about infection of Arabidopsis forfunctional verification and get the T1generation seeds.Analysis of DNA methylation differences in patterns and levels for maize materialsagronomic traits, indicating that the gene methylation heterosis is playing a significant role.