Effects Of Rice And Different Forms Of Brown Rice, Rice Husk In Daily Diet On Intestinal Micro Flora Of Geese

The experiment was conducted to study the effects of rice instead of corn and the effects of different forms of rice husk and brown rice on the intestinal tract microorganisms and cecum VFA, cecum digestive physiology in goslings from4to10weeks, using PCR-DGGE molecular technology and modern experimental method of nutrition. In experiment I, a total of6428-d-old Yangzhou goslings of similar weight were selected and randomly assigned to2groups,32goslings comprised of4replicates with8goslings in each group. In the case of the same trophic level, a group fed with corn-soybean diet and the other as the test group fed with corn-rice-soybean diet. In experiment Ⅱ,128Yangzhou gander (4weeks old) were randomly divided into4groups, each with four repeats (8ones/repeat), fed with the same composition and nutrition diet, only with different size of brown rice and rice hull. Four treatments were:A (whole brown rice-crushed rice husk), B (whole brown rice-whole rice husk), C (crushed rice-crushed rice husk), D (crushed rice-whole rice husk). Two trials were conducted for42days. The results showed that:1. Compared to unstable of traditional gut bacteria cultivation method, molecular biology method of DNA sample extraction, PCR-DGGE parallel bands of quantity and the mobility spectrum is roughly same, and have a well consistency.2. The intestinal microbial flora of jejunum between different intestinal segments were different. Intestinal contents of the different sampling parts have certain influences on the intestinal microflora.3. The corn-paddy-soybean diet dramatically affected intestinal microbial flora. It increased intestinal microflora population of anterior intestinal tract (21:15、19:14), and decreased posterior intestinal tract of geese(22:30). The results may be because of the characteristics of rice nutrition.4. The microbial populations of geese decreased in the group of the whole diet. The results affected intestinal microbial flora of ileum and cecum were the same to the experiment I.5.Compared with the influence of rice husk particle size, the brown rice particle size seems to have more influences on intestinal microflora. The interactive effect of whole grain of husk and whole rice on the intestinal flora order is: ileum> jejunum> duodenal> cecum or rectum. 6. Brown rice and rice husk particle size have little effect on cecal volatile fatty acids in goose cecum; The experiment showed that acetic acid, propionic acid, butyric acid ratio of cecal VFA in goose cecum is about10:2:1. And its mechanism also need further research.7. Brown rice and rice husk particle size have some influence on the cecum intestinal morphology. The villus height、villus width and crypt depth of digestive tract in whole brown rice group and whole rice husk were higher than crushed group (P<0.05), but it has no significant affect on V/C (P>0.05). It was showed that different forms of rice husk and brown rice partly affected the cecum villi of each index, but did not affect the cecum mucosa comprehensive absorption function.