A Study Of Chemical Constituents Volatile Oil And Pharmacological Activities From Twigs Of Myricaria Germanica, A Tibetian Medicine

The twigs of Myricaria germanica, a Tibetian medicine in folk, belonging to thegenus Tamaricaceae, distributed in the Qinghai-tibet plateu and what is used to treat ofarthritis. Modern pharmacological studies showed that Myricaria germanica have theantibacterial effects, inflammation, antifatigue and antioxidation.This study extracted volatile oil from the branches and leaves of Myricariagermanica by the way of steam distillation and optimized the best process, andanalysed thechemical constituents by GC-MS.Further,the bioactivity is studied such asthe antibiosis, antioxidant, anti-inflammatory, analgesic effects and so on.The researchcan afford theoretic basis and technological support for the exploitatioon of Myricariagermanica. The study results are as foIlows：1. The best process of extraction is100mesh，8hours of extraction and2hoursfor soaking，which can achieve the highest yield，for0.18%．The influences of the mainfactors were:size> distilled time=soak time．2.90components were isolated by GC-MS. They can be divided to aliphatichydrocarbons (24components,30.11%), esters (11components,16.6%), aromatic (15components,17.31%), the fatty alcohols(4components,4.26%), ethers(2components,0.5%), ketones (2components,0.28%), aldehydes hydrocarbons (6components,0.96%),aliphatic alcohol(3components,2%),thiazoles(3components,1.75%),pyrans(1compo-nent,0.04%), phenolics(5components,1.55%),aliphatic acids(8components,2.06%),alcohols(2components,1.25%), terpenoid (1component,0.15%), amides(3components,1%).3. The inhibition of the volatile oil on nine common bacterium was discussed byfilter paper method such as Escherichia coli, Bacillus subtilis(Ehrenberg)cohn,Clostridium,Bacillus megaterium,Four associated cocci,Staphyloccocus aureus Rosen-bach,Proteus,Shigella,eight stacked cocci.MIC were separately40%,60%,60%,60%,60%,80%,80%,80%,80%. 4. Take Vc as control group,determined the clearance rates of DPPH, OH andperoxidation of lecithin use spectrophotometric method. DPPH radicals clear capacityof volatile oil and Vc respectively53.12%,49.80%at a concentration of10mg/ml. OHradicals clear capacity of volatile oil and Vc is respectively22.56%,83.03%at aconcentration of10mg/ml.The inhibition rates of volatile oil and Vc on the lipidperoxidation is respectively41.35%,-22.34%at a concentration of10mg/ml.5. ouse as experimental materials have inflammations as follow: the ear swellinginduced by xylene in mice, the hyperplasia of granulation tissue induced by cotton ballimplantation, peritoneal capillary permeability changed by acetic acid. oil dose of22.5μl/kg had prominent anti-inflammatory effect (P<0.01)about those inflammations.Thevolatile oil dose of15μl,7.5μl/kg also had prominent anti-inflammatory effect(P<0.05).6. The analgesic effect was observed by acetic acid twisting and hot platemethods.The results showed that the volatile oil dose of22.5μl,15μl,7.5μl/kg hadimproved the pain threshold (P<0.01),In thermal stimulation experiments, the volatileoil dose of22.5μl,15μl/kg had significantly improved the pain threshold (P<0.001)in30,60,90minites. The volatile oil dose of7.5μl/kg had improved the pain threshold(P<0.05) in90minites.These showed that the volatile oil of Myricaria Germanic hadbetter analgesic effects.