Flax Nuclear Sterility Histochemistry And Related Gene Expression

In the study, the conventional paraffin method was applied to explore the period and process of pollen aborsion in dominant nuclear stertile flax using male fertile and male sterile flax seperated from the dominant nuclear stertile flax line as material. The results showed that abnormal structure and pollen abortion began to appear from the period of tetrad in male sterile anther and pollen abortion process could roughly be devided into four stages:infirst stage, the tapetum seperated from the mideum layer during tetrad; in the second stage, microspores stopped further development; in the third stage, tapetum adhered to microspores and disappeared gradully; and in the fourth stage, the anthers shriveled with no pollens in them. Temperal and spacial expression of male sterility related gene MS2-F in flax anther was studied. Digoxin labeled MS2-F specific cRNAprobe was synthesized in vitro using T7and SP6primers, and was hybridized with flax anther slice. Strong hybridyzation signal was obserbed in the tapetum from the period of tetrad to the early period of microspore development, and no hybridization signal was obserbed during pre-tetrad period, proving that MS2-F was specifically expressed in tapetum during the period of tetred and microspore early development.