Basic Study On The Propagation Technique Of Lysiphlebus Testaceipes Cresson

Aphis medicaginis Koch is an important pest of the forage industry, which cause great economic losses to the livestock industry. This article studies the Lysiphlebus testaceipes Cresson, a parasitoid of A. medicaginis, in order to provide a scientific basis for the biological control of A. medicaginis. In this paper, basic biology of L. testaceipes, parasitic factors on parasitism and indoor propagation techniques are investigated. The results are as follows.1.L. testaceipes typically exhibited18-20generations per year in Hohhot, and of parasitism was highest in July and August.2.In the experiment of parasite to different host instar, A. craccivora (2nd Instar)was the most suitable parasitic object of L. testaceipes r3.At25℃, suppling the20%honey water significantly prolong parasitoid adult life. The most suitable temperature22°C-28°C for L. testaceipes, increase parasitoid adult fecundity.4. The model Na=1.1180N/(1+0.0184N),r=9768, can be drawn that the maximum of A. craccivora parasited by L. testaceipes with24hr is60.71, an the instantaneous attack rate (functional factor) is1.118.5. In the model α=0.1289P-1.1863, r=0.7565, when host density remain constant, P-value enhances with the increasing of parasitoid density, causing α to drop, i.e. the discovered parasitic area diminishes.6.At25±1℃,60%-70%RH,16:8(L:D)photoperiod,4000±5001x, Broad bean plants are used for the rearing of aphids. Aphid colonies are reared on broad bean plant with8-10leaves and when aphids grow to2instar,parasitiod is introduced with (l)parasitiod:(50)aphid, keeping culture condition constant till mummified aphids happened,and then collecting parasitoid adult.