Effects Of N-acetylcysteine On Intestinal Immunological Stress And Antioxidant Capacity Of Piglets Challenged With Lipopolysaccharide

The aim of the current study was to evaluate effects of dietary supplementation with different levels of N-acetylcysteine (NAC) on intestinal mucosal immune stress and antioxidant capactiy of piglets after single lipopolysaccharide (LPS) challenge. Twenty-four healthy piglets with the same genetic origin and similar body weight were randomly allocated into4treatments:(1) control group (fed basal diet, injected physiological saline):(2) LPS group (fed basal diet, injected LPS);(3)250mg/kg NAC group (fed basal diet+250mg/kg NAC. injected LPS):(4)500mg/kg NAC group (fed basal diet+500mg/kg NAC. injected LPS). On d17. the piglets in the LPS,250mg/kg NAC and500mg/kg NAC groups were injected intraperitoneally with100μg/kg BW LPS, whereas the piglets in the control group received the same volume of physiological saline. Blood samples were collected from precava vein at3h post-injection of LPS or saline, and at6h post-injection of LPS or saline, the piglets were killed to collect intestinal mucosa. the effects of NAC on intestinal immune stress and redox status were tested.The results showed as follows.1. Effects of N-acetylcysteine with different levels on intestinal mucosal immune stress of piglets challenged with lipopolysaccharide.The results showed that:(1) Compared with the control group. LPS challenge increased the levels of IL-2. IL-6. and PGE2in duodenum and ileum mucosae (P<0.05). increased the levels of IL-2and PGE2in jejunum mucosa (P<0.05):dietary supplementation of250or500mg/kg NAC reduced the levels of IL-2, IL-6. and PGE2in duodenum and ileum mucosae (P<0.05). and reduced the levels of IL-2and PGE? in jejunum mucosa (P<0.05):(2) Compared with the control group. LPS challenge increased the levels of expression of HSP70in duodenum, jejunum and jejunum mucosae of piglets (P<0.05):dietary supplementation of250or500mg/kg NAC reduced the levels of expression of HSP70in duodenum, jejunum and jejunum mucosae (P<0.05):(3) Compared with the control group. LPS challenge increased the levels of expression of NF-κB in jejunum and ileum mucosa (P<0.05), dietary supplementation of250or500mg/kg NAC reduced the levels of expression of NF-κB(P<0.05).2. Effects of N-acetylcysteine with different levels on on antioxidant capacity of intestinal mucosa of piglets challenged with lipopolysaccharide.The results showed that:(1)Compared with the control group, LPS challenge reduced SOD, CAT and GSH-Px activities in duodenum and jejunum mucosa (P<0.05), reduced SOD and CAT activities in ileum mucosa (P<0.05). reduced SOD activities in plasma (P<0.05); dietary supplementation of250or500mg/kg NAC increased GSH-Px activities in duodenum mucosa (P<0.05). increased CAT and GSH-Px activities in jejunum (P<0.05). increased SOD and CAT activities in ileum mucosa (P<0.05). and increased SOD activities in plasma (P<0.05); dietary supplementation of500mg/kg NAC increased SOD and CAT activities in duodenum mucosa (P<0.05), increased SOD activities in jejunum mucosa (P<0.05). GSH-Px activities in ileum mucosa (P<0.05) and CAT activities in plasma (P<0.05);(2) LPS challenge increased the levels of H2O2in duodenum mucosa (P<0.05), increased the levels of MDA in jejunum mucosa (P<0.05). and increased the levels of MDA. H2O2in ileum mucosa and plasma (<0.05); dietary-supplementation of250or500mg/kg NAC reduced the levels of H2O2in duodenum, ileum mucosa and blood samples(P<0.05). and reduced the levels of MDA in jejunum mucosa and plasma (P<0.05).