Genome-wide Analysis Of LIM Genes And Evolution Pattens In Zea Mays L.

LIM protein is a kind of protein family, which containing a plenty of Cysteineand Histidine, mediating the interaction between protein and protein, containing oneor more than one zinc-finger structures in the molecule, broadly existing inEukaryotes, having an important regulation and control role in development processof cells, situating in cell nucleus or cytoplasm or shuttling between cell nucleus andcell cytoplasm. This kind of family members broadly participate in development,differentiation and regulation, a variety of gene transcription and regulation in manycells, which have an important role in growth and development in both plants andanimals. Thus, it is very important to define the function of LIM gene. In this study,the methods of bioinformatics were used to analyze the structural characteristics andevolutionary relationships of LIM protein in the genome of Zea mays L. andArabidopsis thaliana. Now, the study results were summarized as follows:1．Through search and comparison by a variety of bioinformatics software,13LIM genes were obtained in Zea mays L., and9LIM genes in Arabidopsis thaliana.2．Chromosome Physical Mapping analysis on LIM gene in Zea mays L. andArabidopsis thaliana. was carried out, the results showed: These genes weredistributed uneven in10chromosome of Zea mays L., there was no distribution inchromosome9, the number of LIM genes was the most in chromosome1, which had3. Chromosome2and6respectively had2LIM genes, and others all only had1LIMgene except chromosome9, LIM genes of chromosome1were most in Arabidopsisthaliana, which had3. chromosome2,3and4both had2LIM genes, and there was nodistribution in chromosome5.3．Through the construction of phylogenetic tree, the evolutionary relationship ofLIM gene in Zea mays L. and Arabidopsis thaliana. was analyzed, the results showed:LIM genes in Zea mays L. had2branches, and whose similarity was very significant,compared with that in Arabidopsis thaliana, each branch was composed by the LIMgenes from Zea mays L. and Arabidopsis thaliana except AtLIM4. The number ofLIM genes in Zea mays L. was more than that in Arabidopsis thaliana, this couldillustrated there was gene duplication phenomenon in Zea mays L. Abovephenomenon all illustrated that the evolution of LIM genes in Zea mays L. was morecomplex than that in Arabidopsis thaliana.4．Through the motifs analysis of LIM gene in Zea mays L. and Arabidopsis thaliana. the results showed: The motif structure on LIM gene was complex in Zeamays L., some motif had1453amino acids and a dozen of different motifs, but somemotif had less than100amino acids. The shortest LIM gene only included3motifs.Most genes included motif1, motif2and motif4, and this could illustrated3motifsplayed important role in immune mechanism of pro-free protein.18motifs wereobtained from the LIM gene in Arabidopsis thaliana., through comparison, most LIMgene included motif2.