| This experiment using glycine and cyanamide as the main raw material synthesis Guanidine acetic acid, the best condition combination is studied, in order to Synthesis of Guanidine acetic acid. Then using the synthesis of acetic acid on Feeding Broilers of AA, Studied guanidine acetic acid on broiler growth performance, slaughter performance, immune organ index and blood indexes, This research mainly is divided into two main parts, the test results, the results of their study as follows:Experiment.1Guanidine acetic acid synthesis research on optimal technological parametersThis experiment first by single factor in determining the glycine and cyanamide optimal reaction molar ratio of1:1.0-1.0:1.4, Glycine and water with the weight ratio of1:2.6-1.0:3, reaction temperature80℃-90℃, the reaction of optimal time for70min-90min,The test uses three level four factors of orthogonal experiment design, Research Guanidine acetic acid synthesis temperature, time, reactant molar ratio and between glycine and water weight ratio of acetic acid synthesis size effect. The orthogonal experiment results show, Guanidine acetic acid synthesis of impact factor size is as follows:The Reaction of glycine and water weight ratio, glycine with cyanamide molar ratio, reaction temperature and reaction time. Guanidine acetic acid synthetic optimal combination conditions is:Glycine and cyanamide molar ratio of1:1.2, glycine and water the weight ratio of1:2.8, reaction temperature90℃, reaction time is70min.Experiment.2The influence of Guanidine acetic acid on AA broiler growth performance, slaughter performance and blood indexesIn order to study the effect of Guanidine acetic acid on AA broiler growth performance, slaughter performance and blood indexes.300AA broilers are selected to be randomly divided into5groups; Every group sets up3repetitions, and20chickens in each repetition. test is divided into early (1～21d) and late (22～42d) in two stages, the experimental period of6weeks. The control group was fed with basic diet, test group Ⅰ, Ⅱ, Ⅲ, Ⅳ respectively in based diet supplemented with0.02%,0.04%,0.06%,0.08%of acetic acid. Test results show that, In the production of performance:from the initial point of view, the test group III and group IV in trial production performance compared with other groups, the performance of the more prominent; from the latter view, test group III and group IV in the weight gain test has a better effect, reached extremely significant difference level (P＜0.01); in the during feeding, test group Ⅲ and group IV in the test of production performance and no significant difference. At slaughter performance:the slaughter rate, ratio of breast muscle and leg muscle rate compared with the control group and the experimental group were increased, III and IV compared with the control group have reached extremely significant difference level (P＜0.01), abdominal fat rate decreased, but did not reach the significant level. In the index of immune organs:the thymus index, spleen index and bursal index compared with the control group have improved, but have not reached the significant level. In the blood indexes:Serum total protein in experimental group compared with the control group were increased, serum globulin and albumin as well as A/G content had increasing trend, but the difference was not significant; serum urea nitrogen at the age of21days, the experimental group compared with control levels have decreased, but has not reached the significant level:42days of age. test group IV compared with the control group, serum urea nitrogen content decreased by30.69%(P＜0.05), reached the significant level, the other experimental groups have also declined, but has not reached the significant level. |
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