The Detection Method Of Thiamphenicol In Honeybee By SPE-HPLC

Thiamphenicol belong to amidealcohols antibiotics which has a strong activity on Gram-negative bacteria and Gram-positive bacteria and it has a broad-spectrum antimicrobial ability. EU, US and China has disabled chloramphenicol in animal husbandry and aquaculture because of the side effects of chloramphenicol, In bee breeding process thiamphenicol has become a substitute of chloramphenicol to be used.Although the thiamphenicol has lower residues in animals, the side effects still exist, We need to create a more agile and efficient thiamphenicol drug residue detection technology for the protection of consumer safety and control thiamphenicol residues in bee products. At present, the reports about detection of chloramphenicol in bee products and aquatic products is endless, however, not many detection methods of thiamphenicol in honey and other bee products.A method of determination of thiamphenicol residue in honey by HPLC was developed.The sample was extracted from honey by ethyl acetate, concentrated by rotary evaporator and purification by Cis SPE cartridge, detected at a wavelength of225nm,water:acetonitrile=80:20(V/V) as the mobile phase, at a flow rate1.0ml/min,column temperature is35℃,injection volunm is20μL.The linear ranges were from10μg/g to100μg/g for thiamphenicol and the correlation coefficients were0.9998. The average recoveries for TAP were between71.3%and90.9%.Coefficients of variation ranged from2%to3.4%. The detection limits for thiamphenicol were30μg/kg,less than50μg/kg which was provided by Industry Standard of the People’s Republic of China SN0199-93.The method has good repeatability and high sensitivity, and can be applied for the determination of TAP residue in the honey. It provide a new method to detected thiamphenicol residues and provide an important theoretical and practical basis with monitoring of residues in bee products and protect people’s health.