Studies On Centrifugal Damage Physiology And Disease Resistence Of Mung Bean Spouts

This paper reported on using the laboratory simulation centrifugal bruising mung bean spouts for measurement of effects of centrifugal damage on physiology and disease resistence of mung bean spouts. Firstly, trypan blue staining, neutral red staining, aniline blue staining, sarranine staining and fast green staining were used to find the centrifugal force of disabled cells and to determine whether the cell necrosis was caused by the cell membrane leakage. Secondly, growth vigor and rotten rate of mung bean spouts, combing with the determination of physiological indexes related to membrane lipid peroxidation, reactive oxygen, protective enzyme and enzymatic browning, was to discuss the effects of centrifugal damage. Finally, disease resistence was studied. Growth vigor and rotten rate of mung bean sprout inoculated Penicillium expansium, salicylic acid, lignin and PAL activity, was to evaluate the bascal disease resistence of mung bean spouts at different centrifugal force. Separating and purificating pathogenic bacteria from mung bean sprouts, combing with Oxford cup antibacterial experiment, was to judge the ability of plant potential generating new cells and specific disease resistence. Mung bean sprouts inoculated Penicillium expansium at centrifugal damage (RCF=376×g and RCF=845×g) were processed by methyl jasmonic acid, chitosan, salicylic acid, Vc and lecithin solution, to improve their protective capability. The results were shown as follow:(1) The dyeing experiments indicated that disabled cells might appear between94×g and376×g and cells might be ruptured at3381×g.(2) The results of physiological indexes showed that the integrity of cell membrane was remained at94×g, while it was damaged above376xg. and the stronger centrifugal damage, the faster cell membrane leakage. SOD and CAT activity presented the trend of fluctuation of rise and fall, with POD activity descending, which was beneficial to resist damage."Reactive oxygen burst" appeared after0.5h, and the stronger centrifugal damage, the faster O-2reduced rate. Total hydroxybenzene content was gradually increased, positively correlated with the browning degree. PPO activity was decreased, which might be caused by protease.(3) The disease resistence experimental results revealed that the growth vigor of mung bean sprouts at94×g was similar to the control. As the centrifugal damage aggravated, the browning degree and rotten rate increased. The disease resistence indexes such as salicylic acid, lignin and PAL presented the trend of rise and fall, which demonstrated that early in the mechanical damage, the disease resistence of mung bean spouts had been enhanced. And as time extended and damage increased, its resistance was weakened. Oxford cup antibacterial experiments showed that the antimicrobial components might be some macromolecular substances, such as special protein, etc. In addition, lecithin, salicylic acid and Vc in the test had a better performance in protecting mung bean sprouts in mechanical damage and restraining the decay. But in order to understand the mechanism clearly, the experiment needs further discussion and verification.