Biological Assessments Of Transgenic Cry2A And Cry1C Rice On Ecological Fitness Of No-target Insect Green Leafhopper

This paper take transgenic rice varieties with cry2A or cry1C gene for material, through the laboratory and the field experiments, in order to evaluate the biosafty effects of different transgenic rice with insect resistance on ecological fitness of the non-target insect pest the green leafhopper, and main contents are as follows:1Effects of transgenic cry2A rice and transgenic cry1C rice on the growth and development of the green leafhopperThe feeding, oviposition, growth and development of the green leafhopper, Nephotettix cincticeps (Fabricius) on transgenic ay2A rice (H2A) and transgenic cry1C rice (H1C) are measured, compared with their parental cultivar Minghui63(HOK).The results show that the survival rate in nymph period, female ratio and hatch rate of green leafhopper on HCK, H2A and H1C were close to eacher. And also there were no significant differences on the weight of fresh female/male adult, female/male longevity, oviposition period, fecundity and egg period of the green leafhopper. However, the preoviposition period on H2A and H1C was significantly shortter than that on HCK. And the nymph period on H1C was significantly longer than that on H2A and HCK, either. Also, there was some difference in innate capacity of increase. In selective conditions, the green leafhoppers tend to HCK. But after72hours, percentages of the green leafhopper loading to HCK, H2A and H1C were significant differences. Oviposition behavior of the green leafhopper on HCK, H2A and H1C was no significant difference. After feeding on HCK, H2A and H1C for24h, honeydew excreted by the green leafhopper feeding on H1C was lower than that of HCK, but no significant difference than that of H2A. And there was no no significant difference between H2A and HCK.2Effects of transgenic cry2A rice and transgenic cry1C rice on the population of the green leafhopper under field conditions Field studies were conducted to evaluate the impacts of H2A and H1C on their non-target pest the green leafhopper by the sampling methods of yellow sticky card, compared with their parental control HCK (Minghui63). The result show that population densities and population dynamics of the green leafhopper in H2A and H1C were similar to those in the control, HCK.